Detergent-solubilized Escherichia coli cytochrome bo3 ubiquinol oxidase: A monomeric, not a dimeric complex

Andrej Musatov, Jaime Ortega-Lopez, Borries Demeler, Jeffrey P. Osborne, Robert B. Gennis, Neal C. Robinson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The protein molecular weight, M(r), and hydrodynamic radius, R(s), of Triton X-100-solubilized Escherichia coli cytochrome bo3 were evaluated by computer fitting of sedimentation velocity data with finite element solutions to the Lamm equation. Detergent-solubilized cytochrome bo3 sediments as a homogeneous species with an s(20,w) of 6.75 s and a D(20,w) of 3.71x10-7 cm2/s, corresponding to a R(s) of 5.8 nm and a M(r) of 144 000±3500. The protein molecular weight agrees very well with the value of 143 929 calculated from the four known subunit sequences and the value of 143 025 measured by MALDI mass spectrometry for the histidine-tagged enzyme. We conclude that detergent-solubilized E. coli ubiquinol oxidase is a monomeric complex of the four known subunits. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)153-156
Number of pages4
JournalFEBS Letters
Volume457
Issue number1
DOIs
StatePublished - Aug 20 1999

Fingerprint

Cytochromes
Detergents
Escherichia coli
Molecular Weight
Molecular weight
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Octoxynol
Hydrodynamics
Sedimentation
Histidine
Mass spectrometry
Mass Spectrometry
Sediments
Proteins
Enzymes
ubiquinol oxidase

Keywords

  • Computer analysis
  • Cytochrome bo ubiquinol oxidase
  • Escherichia coli
  • Sedimentation equilibrium
  • Sedimentation velocity

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Musatov, A., Ortega-Lopez, J., Demeler, B., Osborne, J. P., Gennis, R. B., & Robinson, N. C. (1999). Detergent-solubilized Escherichia coli cytochrome bo3 ubiquinol oxidase: A monomeric, not a dimeric complex. FEBS Letters, 457(1), 153-156. https://doi.org/10.1016/S0014-5793(99)01020-0

Detergent-solubilized Escherichia coli cytochrome bo3 ubiquinol oxidase : A monomeric, not a dimeric complex. / Musatov, Andrej; Ortega-Lopez, Jaime; Demeler, Borries; Osborne, Jeffrey P.; Gennis, Robert B.; Robinson, Neal C.

In: FEBS Letters, Vol. 457, No. 1, 20.08.1999, p. 153-156.

Research output: Contribution to journalArticle

Musatov, A, Ortega-Lopez, J, Demeler, B, Osborne, JP, Gennis, RB & Robinson, NC 1999, 'Detergent-solubilized Escherichia coli cytochrome bo3 ubiquinol oxidase: A monomeric, not a dimeric complex', FEBS Letters, vol. 457, no. 1, pp. 153-156. https://doi.org/10.1016/S0014-5793(99)01020-0
Musatov, Andrej ; Ortega-Lopez, Jaime ; Demeler, Borries ; Osborne, Jeffrey P. ; Gennis, Robert B. ; Robinson, Neal C. / Detergent-solubilized Escherichia coli cytochrome bo3 ubiquinol oxidase : A monomeric, not a dimeric complex. In: FEBS Letters. 1999 ; Vol. 457, No. 1. pp. 153-156.
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