Detection systems for carbapenemase gene identification should include the SME serine carbapenemase

Karen Bush, Megan Pannell, John L. Lock, Anne Marie Queenan, James H. Jorgensen, Ryan M. Lee, James S. Lewis, Deidre Jarrett

Research output: Contribution to journalComment/debatepeer-review

29 Scopus citations

Abstract

Carbapenemase detection has become a major problem in hospitals that encounter outbreaks of infections caused by carbapenem-resistant Gram-negative bacteria. Rapid detection systems have been reported using multiplex PCR analyses and DNA microarray assays. Major carbapenemases that are detected by these systems include the KPC and OXA serine carbapenemases, and the IMP, VIM and NDM families of metallo-β-lactamases. However, increasing numbers of the SME serine carbapenemase are being reported from Serratia marcescens, especially from North and South America. These organisms differ from many of the other carbapenemase-producing pathogens in that they are generally susceptible to the expanded-spectrum cephalosporins ceftazidime and cefepime while retaining resistance to almost all other β-lactam antibiotics. Thus, multiplex PCR assays or DNA microarray testing of carbapenem-resistant S. marcescens isolates should include analyses for production of the SME carbapenemase. Confirmation of the presence of this enzyme may provide reassurance that oxyimino- cephalosporins can be considered for treatment of infections caused by these carbapenem-resistant pathogens.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalInternational Journal of Antimicrobial Agents
Volume41
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • Carbapenemase
  • Detection
  • Microarray
  • Multiplex PCR
  • SME

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

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