Detection of Tn917-like sequences within a Tn916-like conjugative transposon (Tn3872) in erythromycin-resistant isolates of Streptococcus pneumoniae

Linda K. McDougal, Fred C. Tenover, Linda N. Lee, J. Kamlle Rasheed, Jan E Patterson, James H. Jorgensen, Donald J. Leblanc

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization. Like Tn1545, Tn917 also encodes an ermAM gene but does not mediate resistance to other antimicrobial agents. Furthermore, nucleotide sequence analyses of the DNAs flanking three of the Tn917-like elements revealed that they were inserted into orf9 of a Tn916-like element in a composite transposon-like structure (Tn3872). Other MLS-resistant strains appeared to contain Tn1545-like elements that had suffered a deletion of sequences including the aphA-3 sequences responsible for kanamycin resistance. Thus, the MLS resistance phenotype in pneumococci appears to be mediated by the ermAM present on a much wider variety of genetic elements than was previously appreciated.

Original languageEnglish (US)
Pages (from-to)2312-2318
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume42
Issue number9
StatePublished - Sep 1998

Fingerprint

Streptogramin B
Lincosamides
Macrolides
Erythromycin
Streptococcus pneumoniae
DNA
Kanamycin Resistance
Sequence Deletion
Anti-Infective Agents
Sequence Analysis
Phenotype
Genes

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Detection of Tn917-like sequences within a Tn916-like conjugative transposon (Tn3872) in erythromycin-resistant isolates of Streptococcus pneumoniae. / McDougal, Linda K.; Tenover, Fred C.; Lee, Linda N.; Rasheed, J. Kamlle; Patterson, Jan E; Jorgensen, James H.; Leblanc, Donald J.

In: Antimicrobial Agents and Chemotherapy, Vol. 42, No. 9, 09.1998, p. 2312-2318.

Research output: Contribution to journalArticle

McDougal, Linda K. ; Tenover, Fred C. ; Lee, Linda N. ; Rasheed, J. Kamlle ; Patterson, Jan E ; Jorgensen, James H. ; Leblanc, Donald J. / Detection of Tn917-like sequences within a Tn916-like conjugative transposon (Tn3872) in erythromycin-resistant isolates of Streptococcus pneumoniae. In: Antimicrobial Agents and Chemotherapy. 1998 ; Vol. 42, No. 9. pp. 2312-2318.
@article{7b92eabda56542a1affd3ddfe6571657,
title = "Detection of Tn917-like sequences within a Tn916-like conjugative transposon (Tn3872) in erythromycin-resistant isolates of Streptococcus pneumoniae",
abstract = "A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization. Like Tn1545, Tn917 also encodes an ermAM gene but does not mediate resistance to other antimicrobial agents. Furthermore, nucleotide sequence analyses of the DNAs flanking three of the Tn917-like elements revealed that they were inserted into orf9 of a Tn916-like element in a composite transposon-like structure (Tn3872). Other MLS-resistant strains appeared to contain Tn1545-like elements that had suffered a deletion of sequences including the aphA-3 sequences responsible for kanamycin resistance. Thus, the MLS resistance phenotype in pneumococci appears to be mediated by the ermAM present on a much wider variety of genetic elements than was previously appreciated.",
author = "McDougal, {Linda K.} and Tenover, {Fred C.} and Lee, {Linda N.} and Rasheed, {J. Kamlle} and Patterson, {Jan E} and Jorgensen, {James H.} and Leblanc, {Donald J.}",
year = "1998",
month = "9",
language = "English (US)",
volume = "42",
pages = "2312--2318",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - Detection of Tn917-like sequences within a Tn916-like conjugative transposon (Tn3872) in erythromycin-resistant isolates of Streptococcus pneumoniae

AU - McDougal, Linda K.

AU - Tenover, Fred C.

AU - Lee, Linda N.

AU - Rasheed, J. Kamlle

AU - Patterson, Jan E

AU - Jorgensen, James H.

AU - Leblanc, Donald J.

PY - 1998/9

Y1 - 1998/9

N2 - A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization. Like Tn1545, Tn917 also encodes an ermAM gene but does not mediate resistance to other antimicrobial agents. Furthermore, nucleotide sequence analyses of the DNAs flanking three of the Tn917-like elements revealed that they were inserted into orf9 of a Tn916-like element in a composite transposon-like structure (Tn3872). Other MLS-resistant strains appeared to contain Tn1545-like elements that had suffered a deletion of sequences including the aphA-3 sequences responsible for kanamycin resistance. Thus, the MLS resistance phenotype in pneumococci appears to be mediated by the ermAM present on a much wider variety of genetic elements than was previously appreciated.

AB - A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization. Like Tn1545, Tn917 also encodes an ermAM gene but does not mediate resistance to other antimicrobial agents. Furthermore, nucleotide sequence analyses of the DNAs flanking three of the Tn917-like elements revealed that they were inserted into orf9 of a Tn916-like element in a composite transposon-like structure (Tn3872). Other MLS-resistant strains appeared to contain Tn1545-like elements that had suffered a deletion of sequences including the aphA-3 sequences responsible for kanamycin resistance. Thus, the MLS resistance phenotype in pneumococci appears to be mediated by the ermAM present on a much wider variety of genetic elements than was previously appreciated.

UR - http://www.scopus.com/inward/record.url?scp=0031660991&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031660991&partnerID=8YFLogxK

M3 - Article

C2 - 9736555

AN - SCOPUS:0031660991

VL - 42

SP - 2312

EP - 2318

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 9

ER -