TY - JOUR
T1 - Detection of reverse transcriptase by a highly sensitive assay in sera from persons infected with human immunodeficiency virus type 1
AU - Heneine, Walid
AU - Yamamoto, Shinji
AU - Switzer, William M.
AU - Spira, Thomas J.
AU - Folks, Thomas M.
PY - 1995/5
Y1 - 1995/5
N2 - In an ultrasensitive assay for reverse transcriptase (RT), an in vitro-transcribed heteropoly-meric RNA sequence was used as a template and polymerase chain reaction (PCR) amplification with Southern blot hybridization served as a detection system for the cDNA reaction product. The assay, called Amp-RT, detected 9 tested retroviruses in unconcentrated culture supernatants diluted 102- to 105-fold. A comparative analysis using human immunodeficiency virus type 1 (HIV-l) revealed that Amp-RT was 100, 000 times more sensitive than the standard RT assay, 10, 000 times more sensitive than p24 antigen capture and branched DNA assays, and 100 times more sensitive than RT-PCR or TCID50assays. Analysis of serum specimens from 42 HIV-l-infected persons by Amp-RT showed that 36 samples (85.7%) were RT-positive. In contrast, 41 serum specimens from persons seronegative for HIV-1 and human T lymphotropic virus types I and II were all Amp-RT-negative.
AB - In an ultrasensitive assay for reverse transcriptase (RT), an in vitro-transcribed heteropoly-meric RNA sequence was used as a template and polymerase chain reaction (PCR) amplification with Southern blot hybridization served as a detection system for the cDNA reaction product. The assay, called Amp-RT, detected 9 tested retroviruses in unconcentrated culture supernatants diluted 102- to 105-fold. A comparative analysis using human immunodeficiency virus type 1 (HIV-l) revealed that Amp-RT was 100, 000 times more sensitive than the standard RT assay, 10, 000 times more sensitive than p24 antigen capture and branched DNA assays, and 100 times more sensitive than RT-PCR or TCID50assays. Analysis of serum specimens from 42 HIV-l-infected persons by Amp-RT showed that 36 samples (85.7%) were RT-positive. In contrast, 41 serum specimens from persons seronegative for HIV-1 and human T lymphotropic virus types I and II were all Amp-RT-negative.
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U2 - 10.1093/infdis/171.5.1210
DO - 10.1093/infdis/171.5.1210
M3 - Article
C2 - 7538549
AN - SCOPUS:0028927515
VL - 171
SP - 1210
EP - 1216
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 5
ER -