Detection of recurrent copy number loss at Yp11.2 involving TSPY gene cluster in prostate cancer using array-based comparative genomic hybridization

Sapna Vijayakumar, Devon C. Hall, Xavier T. Reveles, Dean A. Troyer, Ian M. Thompson, Dawn Garcia, Rui Hua Xiang, Robin J. Leach, Teresa L. Johnson-Pais, Susan L. Naylor

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Prostate cancer is the second leading cause of cancer deaths among American men. The loss of Y chromosome has been frequently observed in primary prostate cancer as well as other types of cancer. Earlier, we showed that introduction of the human Y chromosome suppresses the in vivo tumorigenicity of the prostate cancer cell line PC-3. To further characterize the Y chromosome, we have developed a high-density bacterial artificial chromosome (BAC) microarray containing 178 BAC clones from the human Y chromosome. BAC microarray was used for array comparative genomic hybridization on prostate cancer samples and cell lines. The most prominent observation on prostate cancer specimens was a deletion at Yp11.2 containing the TSPY tandem gene array. Out of 36 primary prostate tumors analyzed, 16 (44.4%) samples exhibited loss of TSPY gene copies. Notably, we observed association between the number of TSPY copies in the blood and the incidence of prostate cancer. Moreover, PC-3 hybrids with an intact Yp11.2 did not grow tumors in nude mice, whereas PC-3 hybrids with a deletion at Yp11.2 grew tumors in nude mice.

Original languageEnglish (US)
Pages (from-to)4055-4064
Number of pages10
JournalCancer Research
Volume66
Issue number8
DOIs
StatePublished - Apr 15 2006

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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