Detection of plaque structure and composition using OCT combined with two-photon luminescence (TPL) imaging

Tianyi Wang, Austin McElroy, David Halaney, Deborah Vela, Edmund Fung, Shafat Hossain, Jennifer Phipps, Bingqing Wang, Biwei Yin, Marc D. Feldman, Thomas E. Milner

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background and Objectives Atherosclerosis and plaque rupture leads to myocardial infarction and stroke. A novel hybrid optical coherence tomography (OCT) and two-photon luminescence (TPL) fiber-based imaging system was developed to characterize tissue constituents in the context of plaque morphology. Study Design/Materials and Methods Ex vivo coronary arteries (34 regions of interest) from three human hearts with atherosclerotic plaques were examined by OCT-TPL imaging. Histological sections (4μm in thickness) were stained with Oil Red O for lipid, Von Kossa for calcium, and Verhoeff-Masson Tri-Elastic for collagen/elastin fibers and compared with imaging results. Results Biochemical components in plaques including lipid, oxidized-LDL, and calcium, as well as a non-tissue component (metal) are distinguished by multi-channel TPL images with statistical significance (P<0.001). TPL imaging provides complementary optical contrast to OCT (two-photon absorption/emission vs scattering). Merged OCT-TPL images demonstrate the distribution of lipid deposits in registration with detailed plaque surface profile. Conclusions Results suggest that multi-channel TPL imaging can effectively identify lipid sub-types and different plaque components. Furthermore, fiber-based hybrid OCT-TPL imaging simultaneously detects plaque structure and composition, improving the efficacy of vulnerable plaque detection and characterization. Lasers Surg. Med. 47:485-494, 2015.

Original languageEnglish (US)
Pages (from-to)485-494
Number of pages10
JournalLasers in Surgery and Medicine
Issue number6
StatePublished - Aug 1 2015


  • atherosclerotic plaque
  • calcium
  • collagen/elastin fiber
  • hybrid imaging
  • lipid
  • optical coherence tomography
  • oxidized-LDL
  • photonic crystal fiber
  • two-photon luminescence imaging

ASJC Scopus subject areas

  • Surgery
  • Dermatology


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