Abstract
Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,3-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring criteria for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 242-249 |
| Number of pages | 8 |
| Journal | Cancer Research |
| Volume | 45 |
| Issue number | 1 |
| State | Published - 1985 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Detection of nuclear anomalies in the colonic epithelium of the mouse. / Blakey, D. H.; Duncan, A. M V; Wargovich, Michael J; Goldberg, M. T.; Bruce, W. R.; Heddle, J. A.
In: Cancer Research, Vol. 45, No. 1, 1985, p. 242-249.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of nuclear anomalies in the colonic epithelium of the mouse
AU - Blakey, D. H.
AU - Duncan, A. M V
AU - Wargovich, Michael J
AU - Goldberg, M. T.
AU - Bruce, W. R.
AU - Heddle, J. A.
PY - 1985
Y1 - 1985
N2 - Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,3-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring criteria for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.
AB - Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,3-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring criteria for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.
UR - http://www.scopus.com/inward/record.url?scp=0021934347&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021934347&partnerID=8YFLogxK
M3 - Article
C2 - 3965135
AN - SCOPUS:0021934347
VL - 45
SP - 242
EP - 249
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 1
ER -