Detection of Nuclear Anomalies in the Colonic Epithelium of the Mouse

D. H. Blakey, A. M.V. Duncan, M. J. Wargovich, M. T. Goldberg, W. R. Bruce, J. A. Heddle

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,2-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring critiera for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.

Original languageEnglish (US)
Pages (from-to)242-249
Number of pages8
JournalCancer Research
Volume45
Issue number1
StatePublished - Jan 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Blakey, D. H., Duncan, A. M. V., Wargovich, M. J., Goldberg, M. T., Bruce, W. R., & Heddle, J. A. (1985). Detection of Nuclear Anomalies in the Colonic Epithelium of the Mouse. Cancer Research, 45(1), 242-249.