Detection of nuclear anomalies in the colonic epithelium of the mouse

D. H. Blakey, A. M V Duncan, Michael J Wargovich, M. T. Goldberg, W. R. Bruce, J. A. Heddle

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,3-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring criteria for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.

Original languageEnglish (US)
Pages (from-to)242-249
Number of pages8
JournalCancer Research
Volume45
Issue number1
StatePublished - 1985
Externally publishedYes

Fingerprint

Carcinogens
Epithelium
Colon
Dimethylhydrazines
Diet

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Blakey, D. H., Duncan, A. M. V., Wargovich, M. J., Goldberg, M. T., Bruce, W. R., & Heddle, J. A. (1985). Detection of nuclear anomalies in the colonic epithelium of the mouse. Cancer Research, 45(1), 242-249.

Detection of nuclear anomalies in the colonic epithelium of the mouse. / Blakey, D. H.; Duncan, A. M V; Wargovich, Michael J; Goldberg, M. T.; Bruce, W. R.; Heddle, J. A.

In: Cancer Research, Vol. 45, No. 1, 1985, p. 242-249.

Research output: Contribution to journalArticle

Blakey, DH, Duncan, AMV, Wargovich, MJ, Goldberg, MT, Bruce, WR & Heddle, JA 1985, 'Detection of nuclear anomalies in the colonic epithelium of the mouse', Cancer Research, vol. 45, no. 1, pp. 242-249.
Blakey DH, Duncan AMV, Wargovich MJ, Goldberg MT, Bruce WR, Heddle JA. Detection of nuclear anomalies in the colonic epithelium of the mouse. Cancer Research. 1985;45(1):242-249.
Blakey, D. H. ; Duncan, A. M V ; Wargovich, Michael J ; Goldberg, M. T. ; Bruce, W. R. ; Heddle, J. A. / Detection of nuclear anomalies in the colonic epithelium of the mouse. In: Cancer Research. 1985 ; Vol. 45, No. 1. pp. 242-249.
@article{0adaebb0fad84220a9e71989c7bdb074,
title = "Detection of nuclear anomalies in the colonic epithelium of the mouse",
abstract = "Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,3-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring criteria for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.",
author = "Blakey, {D. H.} and Duncan, {A. M V} and Wargovich, {Michael J} and Goldberg, {M. T.} and Bruce, {W. R.} and Heddle, {J. A.}",
year = "1985",
language = "English (US)",
volume = "45",
pages = "242--249",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "1",

}

TY - JOUR

T1 - Detection of nuclear anomalies in the colonic epithelium of the mouse

AU - Blakey, D. H.

AU - Duncan, A. M V

AU - Wargovich, Michael J

AU - Goldberg, M. T.

AU - Bruce, W. R.

AU - Heddle, J. A.

PY - 1985

Y1 - 1985

N2 - Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,3-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring criteria for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.

AB - Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,3-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring criteria for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.

UR - http://www.scopus.com/inward/record.url?scp=0021934347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021934347&partnerID=8YFLogxK

M3 - Article

C2 - 3965135

AN - SCOPUS:0021934347

VL - 45

SP - 242

EP - 249

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 1

ER -