Abstract
Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,2-dimethylhydrazine and γ-rays, have been used to determine appropriate techniques, conditions, and scoring critiera for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.
Original language | English (US) |
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Pages (from-to) | 242-249 |
Number of pages | 8 |
Journal | Cancer Research |
Volume | 45 |
Issue number | 1 |
State | Published - Jan 1 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Oncology
- Cancer Research