@article{7971d273b450427fbd87a7b699a2727f,
title = "Detection of nitrous oxide in the neuronal nitric oxide synthase reaction by gas chromatography-mass spectrometry",
abstract = "Using headspace gas chromatography-mass spectrometry, we detected significant amounts of nitrous oxide in the reaction products of the monooxygenase reaction catalyzed by neuronal nitric oxide synthase. Nitrous oxide is a dimerization product of nitroxyl anion; its presence in the reaction products indicates that the nitroxyl anion is a product of the neuronal nitric oxide synthase-catalyzed reaction.",
keywords = "Gas chromatography-mass spectrometry, Nitric oxide, Nitric oxide synthase, Nitroxyl",
author = "Yuzuru Ishimura and Ying, \{Tong Gao\} and Panda, \{Satya P\} and Roman, \{Linda J.\} and Masters, \{Bettie S\} and Weintraub, \{Susan T.\}",
note = "Funding Information: This investigation was supported by NIH Grants (GM52419 and HL30050), Grant AQ-1192 from The Robert A. Welch Foundation to BSSM. The SIM GC/MS experiments were performed in the Institutional Mass Spectrometry Shared Resource Facility funded in part by the San Antonio Cancer Institute (NCI CA54174) and the University of Texas Health Science Center at San Antonio, directed by Dr. Susan Weintraub. The authors wish to thank Drs. Pavel Mart{\'a}sek, Mark Panda, and Christopher Marohnic for their help, discussions, and encouragement. The expert technical assistance of Mr. Thomas Shea is gratefully acknowledged. The invaluable advice of Lynn Hunter of Renaissance Analytical, Inc., regarding parts and assembly of the carrier gas system is also gratefully acknowledged. The authors wish to acknowledge the seminal contributions of Osamu Hayaishi to the oxygenase field upon his co-discovery of enzymes that incorporate the elements of molecular oxygen into organic substrates, a truly pioneering achievement that has set many fields of scientific endeavor into motion.",
year = "2005",
month = dec,
day = "9",
doi = "10.1016/j.bbrc.2005.07.202",
language = "English (US)",
volume = "338",
pages = "543--549",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "1",
}