Detection of nitrous oxide in the neuronal nitric oxide synthase reaction by gas chromatography-mass spectrometry

Yuzuru Ishimura, Tong Gao Ying, Satya P. Panda, Linda J. Roman, Bettie Sue S. Masters, Susan T. Weintraub

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Using headspace gas chromatography-mass spectrometry, we detected significant amounts of nitrous oxide in the reaction products of the monooxygenase reaction catalyzed by neuronal nitric oxide synthase. Nitrous oxide is a dimerization product of nitroxyl anion; its presence in the reaction products indicates that the nitroxyl anion is a product of the neuronal nitric oxide synthase-catalyzed reaction.

Original languageEnglish (US)
Pages (from-to)543-549
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number1
DOIs
StatePublished - Dec 9 2005

Keywords

  • Gas chromatography-mass spectrometry
  • Nitric oxide
  • Nitric oxide synthase
  • Nitroxyl

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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