Detection of nitrous oxide in the neuronal nitric oxide synthase reaction by gas chromatography-mass spectrometry

Yuzuru Ishimura, Tong Gao Ying, Satya P. Panda, Linda J. Roman, Bettie Sue S Masters, Susan E Weintraub

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Using headspace gas chromatography-mass spectrometry, we detected significant amounts of nitrous oxide in the reaction products of the monooxygenase reaction catalyzed by neuronal nitric oxide synthase. Nitrous oxide is a dimerization product of nitroxyl anion; its presence in the reaction products indicates that the nitroxyl anion is a product of the neuronal nitric oxide synthase-catalyzed reaction.

Original languageEnglish (US)
Pages (from-to)543-549
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number1
DOIs
StatePublished - Dec 9 2005

Fingerprint

Nitric Oxide Synthase Type I
Nitrous Oxide
Reaction products
Gas chromatography
Gas Chromatography-Mass Spectrometry
Anions
Mass spectrometry
Dimerization
Mixed Function Oxygenases
nitroxyl

Keywords

  • Gas chromatography-mass spectrometry
  • Nitric oxide
  • Nitric oxide synthase
  • Nitroxyl

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Detection of nitrous oxide in the neuronal nitric oxide synthase reaction by gas chromatography-mass spectrometry. / Ishimura, Yuzuru; Ying, Tong Gao; Panda, Satya P.; Roman, Linda J.; Masters, Bettie Sue S; Weintraub, Susan E.

In: Biochemical and Biophysical Research Communications, Vol. 338, No. 1, 09.12.2005, p. 543-549.

Research output: Contribution to journalArticle

Ishimura, Yuzuru ; Ying, Tong Gao ; Panda, Satya P. ; Roman, Linda J. ; Masters, Bettie Sue S ; Weintraub, Susan E. / Detection of nitrous oxide in the neuronal nitric oxide synthase reaction by gas chromatography-mass spectrometry. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 338, No. 1. pp. 543-549.
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