Detection of Leishmania RNA virus 1 proteins

T. L. Cadd; M. C. Keenan; J. L. Patterson

doi:10.1128/jvi.67.9.5647-5650.1993

Detection of Leishmania RNA virus 1 proteins

T. L. Cadd, M. C. Keenan, J. L. Patterson

Research output: Contribution to journal › Comment/debate › peer-review

30 Scopus citations

Abstract

Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.

Original language	English (US)
Pages (from-to)	5647-5650
Number of pages	4
Journal	Journal of virology
Volume	67
Issue number	9
DOIs	https://doi.org/10.1128/jvi.67.9.5647-5650.1993
State	Published - 1993
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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10.1128/jvi.67.9.5647-5650.1993

Cite this

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title = "Detection of Leishmania RNA virus 1 proteins",

abstract = "Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.",

author = "Cadd, {T. L.} and Keenan, {M. C.} and Patterson, {J. L.}",

year = "1993",

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AU - Cadd, T. L.

AU - Keenan, M. C.

AU - Patterson, J. L.

PY - 1993

Y1 - 1993

N2 - Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.

AB - Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.

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U2 - 10.1128/jvi.67.9.5647-5650.1993

DO - 10.1128/jvi.67.9.5647-5650.1993

M3 - Comment/debate

C2 - 8350417

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JO - Journal of Virology

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ER -

Detection of Leishmania RNA virus 1 proteins

Abstract

ASJC Scopus subject areas

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