Detection of Leishmania RNA virus 1 proteins

T. L. Cadd, M. C. Keenan, J. L. Patterson

Research output: Contribution to journalComment/debatepeer-review

30 Scopus citations

Abstract

Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.

Original languageEnglish (US)
Pages (from-to)5647-5650
Number of pages4
JournalJournal of virology
Volume67
Issue number9
DOIs
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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