Detection of Leishmania RNA virus 1 proteins

T. L. Cadd, M. C. Keenan, J. L. Patterson

    Research output: Contribution to journalComment/debatepeer-review

    30 Scopus citations

    Abstract

    Polyclonal antiserum was raised against the peak viral fraction of a sucrose gradient from LRV1-4-infected cells and used in Western immunoblot analysis to identify viral proteins from various isolates. Consistent with this result, in vitro-translated protein from cloned RNA was immunoprecipitated with the same antiserum. The putative capsid at times appeared as a doublet; relative amounts of the two species varied, depending on the method of purification.

    Original languageEnglish (US)
    Pages (from-to)5647-5650
    Number of pages4
    JournalJournal of virology
    Volume67
    Issue number9
    DOIs
    StatePublished - 1993

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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