Abstract
Infectious human immunodeficiency virus type 1 (HIV-1) is difficult to detect in female genital secretions by standard virus culture techniques. To improve detection of cell-free HIV-1 in female genital secretions, we adapted a short-term assay that uses the multinuclear-activation galactosidase indicator (MAGI) assay. When vaginal lavages from HIV-1-infected women were tested with the adapted MAGI assay, 25 (64%) of 39 lavages with detectable, cell-free HIV-1 RNA were shown to have infectious virus. No infectious virus was found in 10 vaginal lavages from HIV-1-infected women with undetectable vaginal viral loads. Significantly (P < 0.01) more lavages from HIV-1-infected women tested positive for infectious virus by the MAGI assay than by standard peripheral blood mononuclear cell (PBMC) coculture, which detected infectious virus in only 6 (17%) of 35 vaginal lavages. Lavages with viral loads of >10,000 copies per lavage yielded significantly (P < 0.01) more positive cultures than those with <10,000 copies by using the MAGI assay. Detection of infectious HIV-1 in vaginal lavages was not associated with the presence of genital tract infections or CD4+-T-cell counts. However, although the results were not significant (P = 0.08), the MAGI assay detected infectious virus from more vaginal lavages at a vaginal pH of ≥4.5 than at a pH of <4.5. These results indicate that the MAGI assay is more sensitive than PBMC culture methods for detecting infectious virus in female genital secretions. Accurate measurements of infectious virus in genital secretions will improve studies that evaluate sexual transmission of HIV-1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4081-4088 |
| Number of pages | 8 |
| Journal | Journal of Clinical Microbiology |
| Volume | 41 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1 2003 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Microbiology (medical)
- Microbiology
Cite this
Detection of infectious human immunodeficiency virus type 1 in female genital secretions by a short-term culture method. / Cummins, James E.; Villanueva, Julie M.; Evans-Strickfaden, Tammy; Sesay, Shekou M.; Abner, Sheila R.; Bush, Timothy J.; Green, Timothy A.; Lennox, Jeffrey L.; Wright, Thomas; Folks, Thomas M.; Hart, Clyde E.; Dezzutti, Charlene S.
In: Journal of Clinical Microbiology, Vol. 41, No. 9, 01.09.2003, p. 4081-4088.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of infectious human immunodeficiency virus type 1 in female genital secretions by a short-term culture method
AU - Cummins, James E.
AU - Villanueva, Julie M.
AU - Evans-Strickfaden, Tammy
AU - Sesay, Shekou M.
AU - Abner, Sheila R.
AU - Bush, Timothy J.
AU - Green, Timothy A.
AU - Lennox, Jeffrey L.
AU - Wright, Thomas
AU - Folks, Thomas M.
AU - Hart, Clyde E.
AU - Dezzutti, Charlene S.
PY - 2003/9/1
Y1 - 2003/9/1
N2 - Infectious human immunodeficiency virus type 1 (HIV-1) is difficult to detect in female genital secretions by standard virus culture techniques. To improve detection of cell-free HIV-1 in female genital secretions, we adapted a short-term assay that uses the multinuclear-activation galactosidase indicator (MAGI) assay. When vaginal lavages from HIV-1-infected women were tested with the adapted MAGI assay, 25 (64%) of 39 lavages with detectable, cell-free HIV-1 RNA were shown to have infectious virus. No infectious virus was found in 10 vaginal lavages from HIV-1-infected women with undetectable vaginal viral loads. Significantly (P < 0.01) more lavages from HIV-1-infected women tested positive for infectious virus by the MAGI assay than by standard peripheral blood mononuclear cell (PBMC) coculture, which detected infectious virus in only 6 (17%) of 35 vaginal lavages. Lavages with viral loads of >10,000 copies per lavage yielded significantly (P < 0.01) more positive cultures than those with <10,000 copies by using the MAGI assay. Detection of infectious HIV-1 in vaginal lavages was not associated with the presence of genital tract infections or CD4+-T-cell counts. However, although the results were not significant (P = 0.08), the MAGI assay detected infectious virus from more vaginal lavages at a vaginal pH of ≥4.5 than at a pH of <4.5. These results indicate that the MAGI assay is more sensitive than PBMC culture methods for detecting infectious virus in female genital secretions. Accurate measurements of infectious virus in genital secretions will improve studies that evaluate sexual transmission of HIV-1.
AB - Infectious human immunodeficiency virus type 1 (HIV-1) is difficult to detect in female genital secretions by standard virus culture techniques. To improve detection of cell-free HIV-1 in female genital secretions, we adapted a short-term assay that uses the multinuclear-activation galactosidase indicator (MAGI) assay. When vaginal lavages from HIV-1-infected women were tested with the adapted MAGI assay, 25 (64%) of 39 lavages with detectable, cell-free HIV-1 RNA were shown to have infectious virus. No infectious virus was found in 10 vaginal lavages from HIV-1-infected women with undetectable vaginal viral loads. Significantly (P < 0.01) more lavages from HIV-1-infected women tested positive for infectious virus by the MAGI assay than by standard peripheral blood mononuclear cell (PBMC) coculture, which detected infectious virus in only 6 (17%) of 35 vaginal lavages. Lavages with viral loads of >10,000 copies per lavage yielded significantly (P < 0.01) more positive cultures than those with <10,000 copies by using the MAGI assay. Detection of infectious HIV-1 in vaginal lavages was not associated with the presence of genital tract infections or CD4+-T-cell counts. However, although the results were not significant (P = 0.08), the MAGI assay detected infectious virus from more vaginal lavages at a vaginal pH of ≥4.5 than at a pH of <4.5. These results indicate that the MAGI assay is more sensitive than PBMC culture methods for detecting infectious virus in female genital secretions. Accurate measurements of infectious virus in genital secretions will improve studies that evaluate sexual transmission of HIV-1.
UR - http://www.scopus.com/inward/record.url?scp=0042421545&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042421545&partnerID=8YFLogxK
U2 - 10.1128/JCM.41.9.4081-4088.2003
DO - 10.1128/JCM.41.9.4081-4088.2003
M3 - Article
C2 - 12958229
AN - SCOPUS:0042421545
VL - 41
SP - 4081
EP - 4088
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 9
ER -