Detection of human T-lymphotropic virus type-I/II env antibodies by immunoassays using recombinant fusion proteins

Donna L. Rudolph, Rima F. Khabbaz, Thomas M. Folks, Renu B. Lal

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    2 Scopus citations

    Abstract

    Two recombinant fusion proteins representing the C-terminus of the envelope glycoprotein of HTLV-I (rEnv-93201-440) and the C-terminus of the external glycoprotein (RE-3165-306) were tested in a Western blot (WB) assay for their ability to detect the presence of env antibodies in serum specimens from HTLV-I (n = 27) and HTLV-II (n = 22) infected individuals. The rEnv-93 reacted with 27 (100%) of 27 HTLV-I-in-fected specimens and 19 (86%) of 22 of HTLV-II-infected specimens. In contrast, RE-3 reacted with 25 (93%) of 27 HTLV-I-infected specimens, and only six (27%) of 22 HTLV-II-infected specimens, thus demonstrating predominant reactivity with HTLV-I compared with HTLV-II. Because of the high sensitivity of rEnv-93 reactivity in both HTLV-I and HTLV-II, the specificity of this env protein was evaluated in specimens with isolated gag reactivity (HTLVind). Of the 44 HTLVind specimens, four (9%) demonstrated reactivity to rEnv-93 in WB assay. We, therefore, conclude that although rEnv-93 is highly sensitive for detection of env protein, it has the potential to yield some false-positive reactions presumably due to the conserved nature of retroviral transmembrane epitopes.

    Original languageEnglish (US)
    Pages (from-to)35-39
    Number of pages5
    JournalDiagnostic Microbiology and Infectious Disease
    Volume17
    Issue number1
    StatePublished - Jul 1 1993

    ASJC Scopus subject areas

    • Microbiology (medical)
    • Infectious Diseases

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