Two recombinant fusion proteins representing the C-terminus of the envelope glycoprotein of HTLV-I (rEnv-93201-440) and the C-terminus of the external glycoprotein (RE-3165-306) were tested in a Western blot (WB) assay for their ability to detect the presence of env antibodies in serum specimens from HTLV-I (n = 27) and HTLV-II (n = 22) infected individuals. The rEnv-93 reacted with 27 (100%) of 27 HTLV-I-in-fected specimens and 19 (86%) of 22 of HTLV-II-infected specimens. In contrast, RE-3 reacted with 25 (93%) of 27 HTLV-I-infected specimens, and only six (27%) of 22 HTLV-II-infected specimens, thus demonstrating predominant reactivity with HTLV-I compared with HTLV-II. Because of the high sensitivity of rEnv-93 reactivity in both HTLV-I and HTLV-II, the specificity of this env protein was evaluated in specimens with isolated gag reactivity (HTLVind). Of the 44 HTLVind specimens, four (9%) demonstrated reactivity to rEnv-93 in WB assay. We, therefore, conclude that although rEnv-93 is highly sensitive for detection of env protein, it has the potential to yield some false-positive reactions presumably due to the conserved nature of retroviral transmembrane epitopes.
|Original language||English (US)|
|Number of pages||5|
|Journal||Diagnostic Microbiology and Infectious Disease|
|State||Published - Jul 1 1993|
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases