Detection of human ADCC activity using automated flow cytometry

A. M. Attallah, P. D. Noguchi, T. Folks, C. T. Noguchi

    Research output: Contribution to journalArticle

    3 Scopus citations

    Abstract

    Using automated flow cytometry techniques we have developed a rapid assay to measure human antibody-dependent cell-mediated cytotoxicity (ADCC). By staining cell cultures for DNA content, chick red blood cell targets can be readily distinguished from human effector cells and ADCC can be measured by changes in their relative proportions. The sensitivity and rapidity of the assay is shown by the finding that at an effector to target ratio of only 2:1, 42% killing can be detected after 1 h incubation.

    Original languageEnglish (US)
    Pages (from-to)124-125
    Number of pages2
    JournalExperientia
    Volume36
    Issue number1
    DOIs
    StatePublished - Jan 1 1980

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Attallah, A. M., Noguchi, P. D., Folks, T., & Noguchi, C. T. (1980). Detection of human ADCC activity using automated flow cytometry. Experientia, 36(1), 124-125. https://doi.org/10.1007/BF02004013