Detection of DNA methylation by MeDIP and MBDCap assays: An overview of techniques

Hang Kai Hsu, Yu I. Weng, Pei Yin Hsu, Tim H.M. Huang, Yi Wen Huang

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

DNA methylation has been characterized as the representative example of epigenetic modifications and implicated in numerous biological processes, such as genomic imprinting and X chromosome inactivation. It primarily occurs at CpG dinucleotides in mammals and plays a critical role in transcriptional regulations. Examination of DNA methylation patterns in gene(s) or across a genome is vital to understand the role of epigenetic modulation in the progress of development and tumorigenesis. Currently, lots of approaches have been developed to investigate DNA methylation patterns for either limited regions or for genomescale studies, but some of them rely on using restriction enzymes. In this chapter, we describe two commonly used protocols to detect enrichment of methylated DNA regions, namely, methylated DNA immunoprecipitation (MeDIP) and capture of methylated DNA by methyl-CpG binding domain-based (MBD) proteins (MBDCap). They are the most economical and effective methods to study DNA methylation either at a single locus or in genome-wide scale.

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalMethods in Molecular Biology
Volume1105
DOIs
StatePublished - Jan 1 2014

Keywords

  • 5-Methylcytosine
  • CpG island
  • DNA methylation
  • MBDCap
  • MeDIP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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