Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation

A. M. Attallah; T. Folks; P. D. Noguchi; T. Noguchi

doi:10.1016/0022-1759(80)90137-4

Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation

A. M. Attallah, T. Folks, P. D. Noguchi, T. Noguchi

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for ⁵¹Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and ⁵¹Cr release.

Original language	English (US)
Pages (from-to)	325-333
Number of pages	9
Journal	Journal of Immunological Methods
Volume	36
Issue number	3-4
DOIs	https://doi.org/10.1016/0022-1759(80)90137-4
State	Published - 1980
Externally published	Yes

Keywords

ADCC
CRBC
Cr
E: T
FCM
antibody-dependent cell-mediated cytotoxicity
chicken red blood cells
chromium isotope 51
effector-to-target
flow cytometry

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(80)90137-4

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Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation. / Attallah, A. M.; Folks, T.; Noguchi, P. D.; Noguchi, T.

In: Journal of Immunological Methods, Vol. 36, No. 3-4, 1980, p. 325-333.

Research output: Contribution to journal › Article › peer-review

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title = "Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation",

abstract = "Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for 51Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and 51Cr release.",

keywords = "ADCC, CRBC, Cr, E: T, FCM, antibody-dependent cell-mediated cytotoxicity, chicken red blood cells, chromium isotope 51, effector-to-target, flow cytometry",

author = "Attallah, {A. M.} and T. Folks and Noguchi, {P. D.} and T. Noguchi",

note = "Funding Information: Division of Pathology, Bureau of Biologics, Food and Drug Administration, the Naval Medical Research Institute, and the Laboratory of Chemical Biology, National Institutes of Arthritis, Metabolic, and Digestive Diseases, National Institutes of Health, Bethesda, MD 20205, U.S.A.",

year = "1980",

doi = "10.1016/0022-1759(80)90137-4",

language = "English (US)",

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AU - Attallah, A. M.

AU - Folks, T.

AU - Noguchi, P. D.

AU - Noguchi, T.

N1 - Funding Information: Division of Pathology, Bureau of Biologics, Food and Drug Administration, the Naval Medical Research Institute, and the Laboratory of Chemical Biology, National Institutes of Arthritis, Metabolic, and Digestive Diseases, National Institutes of Health, Bethesda, MD 20205, U.S.A.

PY - 1980

Y1 - 1980

N2 - Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for 51Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and 51Cr release.

AB - Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for 51Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and 51Cr release.

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KW - CRBC

KW - Cr

KW - E: T

KW - FCM

KW - antibody-dependent cell-mediated cytotoxicity

KW - chicken red blood cells

KW - chromium isotope 51

KW - effector-to-target

KW - flow cytometry

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JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 3-4

ER -

Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation

Abstract

Keywords

ASJC Scopus subject areas

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