Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation

A. M. Attallah; T. Folks; P. D. Noguchi; T. Noguchi

doi:10.1016/0022-1759(80)90137-4

Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation

A. M. Attallah, T. Folks, P. D. Noguchi, T. Noguchi

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for ⁵¹Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and ⁵¹Cr release.

Original language	English (US)
Pages (from-to)	325-333
Number of pages	9
Journal	Journal of Immunological Methods
Volume	36
Issue number	3-4
DOIs	https://doi.org/10.1016/0022-1759(80)90137-4
State	Published - Jan 1 1980

Keywords

ADCC
CRBC
Cr
E: T
FCM
antibody-dependent cell-mediated cytotoxicity
chicken red blood cells
chromium isotope 51
effector-to-target
flow cytometry

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation. / Attallah, A. M.; Folks, T.; Noguchi, P. D.; Noguchi, T.

In: Journal of Immunological Methods, Vol. 36, No. 3-4, 01.01.1980, p. 325-333.

Research output: Contribution to journal › Article › peer-review

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abstract = "Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for 51Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and 51Cr release.",

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Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation

Abstract

Keywords

ASJC Scopus subject areas

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