Abstract
Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for 51Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and 51Cr release.
Original language | English (US) |
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Pages (from-to) | 325-333 |
Number of pages | 9 |
Journal | Journal of Immunological Methods |
Volume | 36 |
Issue number | 3-4 |
DOIs | |
State | Published - Jan 1 1980 |
Keywords
- ADCC
- CRBC
- Cr
- E: T
- FCM
- antibody-dependent cell-mediated cytotoxicity
- chicken red blood cells
- chromium isotope 51
- effector-to-target
- flow cytometry
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology