Abstract
Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for 51Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and 51Cr release.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 325-333 |
| Number of pages | 9 |
| Journal | Journal of Immunological Methods |
| Volume | 36 |
| Issue number | 3-4 |
| State | Published - 1980 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biotechnology
- Immunology
Cite this
Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation. / Attallah, A. M.; Folks, T.; Noguchi, P. D.; Noguchi, T.
In: Journal of Immunological Methods, Vol. 36, No. 3-4, 1980, p. 325-333.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of antibody-dependent cell-mediated cytotoxicity by automated flow cytometry. Comparison to a chromium release assay and characterization of the effector cell subpopulation
AU - Attallah, A. M.
AU - Folks, T.
AU - Noguchi, P. D.
AU - Noguchi, T.
PY - 1980
Y1 - 1980
N2 - Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for 51Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and 51Cr release.
AB - Antibody-dependent cell-mediated cytotoxicity (ADCC) against chick red blood cells (CRBC) can be detected by flow cytometric (FCM) analysis of cellular DNA content. When compared to a standard chromium release assay FCM analysis shows several advantages: (1) equivalent cytotoxicity can be detected after 1 h compared to 4 h for 51Cr; (2) equivalent cytotoxicity can be seen at a 5-fold lower effector-to-target ratio; and (3) no radiolabeling is needed. When mouse spleen cells were fractionated based on adherence to the plastic, adherent cells showed the highest ADCC by both FCM and 51Cr release.
UR - http://www.scopus.com/inward/record.url?scp=0018898748&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018898748&partnerID=8YFLogxK
M3 - Article
C2 - 7000911
AN - SCOPUS:0018898748
VL - 36
SP - 325
EP - 333
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 3-4
ER -