Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays

Robert Mabry, Kathleen Brasky, Robert Geiger, Ricardo Carrion, Gene B. Hubbard, Stephen Leppla, Jean L. Patterson, George Georgiou, B. L. Iverson

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Several strategies that target anthrax toxin are being developed as therapies for infection by Bacillus anthracis. Although the action of the tripartite anthrax toxin has been extensively studied in vitro, relatively little is known about the presence of toxins during an infection in vivo. We developed a series of sensitive sandwich enzyme-linked immunosorbent assays (ELISAs) for detection of both the protective antigen (PA) and lethal factor (LF) components of the anthrax exotoxin in serum. The assays utilize as capture agents an engineered high-affinity antibody to PA, a soluble form of the extracellular domain of the anthrax toxin receptor (ANTXR2/CMG2), or PA itself. Sandwich immunoassays were used to detect and quantify PA and LF in animals infected with the Ames or Vollum strains of anthrax spores. PA and LF were detected before and after signs of toxemia were observed, with increasing levels reported in the late stages of the infection. These results represent the detection of free PA and LF by ELISA in the systemic circulation of two animal models exposed to either of the two fully virulent strains of anthrax. Simple anthrax toxin detection ELISAs could prove useful in the evaluation of potential therapies and possibly as a clinical diagnostic to complement other strategies for the rapid identification of B. anthracis infection.

Original languageEnglish (US)
Pages (from-to)671-677
Number of pages7
JournalClinical and Vaccine Immunology
Volume13
Issue number6
DOIs
StatePublished - Jun 2006
Externally publishedYes

Fingerprint

Bacillus anthracis
Bacilli
Immunoassay
Animals
Antigens
Immunosorbents
Assays
Serum
Anthrax
Enzyme-Linked Immunosorbent Assay
Infection
Enzymes
Toxemia
Exotoxins
Antibody Affinity
Spores
anthrax toxin
Animal Models
Antibodies
Therapeutics

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Mabry, R., Brasky, K., Geiger, R., Carrion, R., Hubbard, G. B., Leppla, S., ... Iverson, B. L. (2006). Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays. Clinical and Vaccine Immunology, 13(6), 671-677. https://doi.org/10.1128/CVI.00023-06

Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays. / Mabry, Robert; Brasky, Kathleen; Geiger, Robert; Carrion, Ricardo; Hubbard, Gene B.; Leppla, Stephen; Patterson, Jean L.; Georgiou, George; Iverson, B. L.

In: Clinical and Vaccine Immunology, Vol. 13, No. 6, 06.2006, p. 671-677.

Research output: Contribution to journalArticle

Mabry, R, Brasky, K, Geiger, R, Carrion, R, Hubbard, GB, Leppla, S, Patterson, JL, Georgiou, G & Iverson, BL 2006, 'Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays', Clinical and Vaccine Immunology, vol. 13, no. 6, pp. 671-677. https://doi.org/10.1128/CVI.00023-06
Mabry, Robert ; Brasky, Kathleen ; Geiger, Robert ; Carrion, Ricardo ; Hubbard, Gene B. ; Leppla, Stephen ; Patterson, Jean L. ; Georgiou, George ; Iverson, B. L. / Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays. In: Clinical and Vaccine Immunology. 2006 ; Vol. 13, No. 6. pp. 671-677.
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