Detection and characterization of agarose-binding, capsid-like particles produced during assembly of a bacteriophage T7 procapsid

Philip Serwer, R. H. Watson, S. J. Hayes

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

It has previously been shown that: (i) during infection of its host, the DNA bacteriophage T7 assembles a DNA-free procapsid (capsid I), a capsid with an envelope differing physically and chemically from the capsid of the mature bacteriophage, and (ii) capsid I converts to a capsid (capsid II) with a bacteriophage-like envelope as it packages DNA. Lysates of phage T7-infected Escherichia coli contained a particle (AG particle) which copurified with capsid II during buoyant density sedimentation, velocity sedimentation, and solid support-free electrophoresis, but was distinguished from capsid II by its apparent diversity during electrophoresis in agarose gels. Treatment of AG particles with trypsin converted most of them to particles that comigrated with trypsin-treated capsid II during electrophoresis in agarose gels. Irreversible binding of AG particles to agarose gels was shown to contribute to the apparent diversity of AG particles during agarose gel electrophoresis. The results of quantitation of AG particles and of capsid I and capsid II in lysates of a nonpermissive host infected with T7 amber mutants suggested that in spite of their capsid II-like properties, most AG particles were produced during assembly of capsid I and not during DNA packaging. The presence of AG particles in T7 lysates explains contradictions in previous data concerning the pathway of T7 assembly.

Original languageEnglish (US)
Pages (from-to)583-594
Number of pages12
JournalJournal of Virology
Volume42
Issue number2
StatePublished - 1982

Fingerprint

Bacteriophage T7
capsid
Capsid
Sepharose
agarose
bacteriophages
Agar Gel Electrophoresis
electrophoresis
gels
virus assembly
Bacteriophages
Trypsin
trypsin
DNA
DNA Packaging
Amber
amber
gel electrophoresis
Electrophoresis

ASJC Scopus subject areas

  • Immunology

Cite this

Detection and characterization of agarose-binding, capsid-like particles produced during assembly of a bacteriophage T7 procapsid. / Serwer, Philip; Watson, R. H.; Hayes, S. J.

In: Journal of Virology, Vol. 42, No. 2, 1982, p. 583-594.

Research output: Contribution to journalArticle

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