The purpose of this study was to determine the viability of the hamster pineal gland in organ culture and to test the effect of norepinephrine (NE) on [3H]serotonin derivatives. In this study, elevated levels of melatonin (7-fold, p<.05), 5-hydroxytrytophol (5-fold, p<.001), 5-methoxytryptophol (1.78-fold, p<.05), and depressed levels of 5-hydroxyindoleacetic acid (3.8-fold, p<.02) and methoxyindoleacetic acid (1.78-fold, p<.05) were detected in the glands following the addition of NE to the medium. In a separate experiment, melatonin concentration in the media was also periodically measured by radioimmunoassay to determine the viability of the organ culture over a four-day period. The melatonin level on day 2 (2321 ± 106 pg/gland) was significantly higher (p<0.01) than on day 3 (1542 ± 86 pg/gland) or day 4 (805 ± 39 pg/gland). The results of these experiments verify the viability of the hamster pineal organ culture and show that the gland responds to NE in vitro.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)