Derivatives of [3H]serotonin in organ cultures of hamster pineal gland

Cheryl M. Craft, Russel J. Reiter

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The purpose of this study was to determine the viability of the hamster pineal gland in organ culture and to test the effect of norepinephrine (NE) on [3H]serotonin derivatives. In this study, elevated levels of melatonin (7-fold, p<.05), 5-hydroxytrytophol (5-fold, p<.001), 5-methoxytryptophol (1.78-fold, p<.05), and depressed levels of 5-hydroxyindoleacetic acid (3.8-fold, p<.02) and methoxyindoleacetic acid (1.78-fold, p<.05) were detected in the glands following the addition of NE to the medium. In a separate experiment, melatonin concentration in the media was also periodically measured by radioimmunoassay to determine the viability of the organ culture over a four-day period. The melatonin level on day 2 (2321 ± 106 pg/gland) was significantly higher (p<0.01) than on day 3 (1542 ± 86 pg/gland) or day 4 (805 ± 39 pg/gland). The results of these experiments verify the viability of the hamster pineal organ culture and show that the gland responds to NE in vitro.

Original languageEnglish (US)
Pages (from-to)1775-1782
Number of pages8
JournalLife Sciences
Volume34
Issue number18
DOIs
StatePublished - Apr 30 1984

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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