Derivation and characterization of the human embryonic stem cell line CR-4: Differentiation to human retinal pigment epithelial cells

John L. Mazzilli, Aleksey Y. Domozhirov, Stacey L. Mueller-Ortiz, Charles A. Garcia, Rick A. Wetsel, Eva M. Zsigmond

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The CR-4 human embryonic stem cell line was derived from the inner cell mass of a developing blastocyst. This cell line has been adapted to grow in feeder-free conditions and is especially well-suited for differentiation to retinal pigment epithelium. The line demonstrates a normal human 46,XX female karyotype. Pluripotency was assessed through qRT-PCR for expression of NANOG, OCT-4, and SOX-2. A teratoma assay was performed and results were positive for all three germ layers. Testing for Mycoplasma was negative.

Original languageEnglish (US)
Pages (from-to)37-40
Number of pages4
JournalStem Cell Research
Volume18
DOIs
StatePublished - Jan 2017
Externally publishedYes

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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