TY - JOUR
T1 - Denture stomatitis
T2 - A role for Candida biofilms
AU - Ramage, Gordon
AU - Tomsett, Kelley
AU - Wickes, Brian L.
AU - López-Ribot, José L.
AU - Redding, Spencer W.
N1 - Funding Information:
This work was partially supported by grant ATP 3659-0080 from the Texas Higher Education Coordinating Board (Advance Technology Program, Biomedicine). J.L.L-R. is the recipient of a New Investigator Award in Molecular Pathogenic Mycology from the Burroughs Wellcome Fund. K.L.T acknowledges the receipt of an American College of Prosthodontists/Proctor and Gamble Fellowship in Complete Denture Prosthodontics. We thank Peggy Miller for assistance in SEM experiments. We thank William Kirkpatrick for processing of microbiological samples.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2004/7
Y1 - 2004/7
N2 - Objective To assess the contribution of Candida biofilms to the etiology of denture stomatitis. Study design Samples of denture acrylic were retrieved from patients with denture stomatitis and subjected to scanning electron microscopy (SEM) analysis. Oral swab and swish samples were taken from the same group of patients and representative C albicans isolates recovered were used to investigate the kinetics of biofilm development in vitro. Results Candida biofilms could be visualized by SEM directly from denture samples from patients with denture stomatitis. These biofilms showed a propensity to adhere along cracks and imperfections of the denture acrylic. C albicans clinical isolates were able to form biofilms in vitro, although differences in the extent of biofilm formation were observed for different isolates recovered from the same patient. Susceptibility testing indicated that the resulting biofilms showed increased resistance to antifungal treatment. Presence of serum and saliva conditioning films increased the initial adherence of selected isolates but had little effect in overall biofilm formation. Conclusions Candida biofilms play a role in denture stomatitis.
AB - Objective To assess the contribution of Candida biofilms to the etiology of denture stomatitis. Study design Samples of denture acrylic were retrieved from patients with denture stomatitis and subjected to scanning electron microscopy (SEM) analysis. Oral swab and swish samples were taken from the same group of patients and representative C albicans isolates recovered were used to investigate the kinetics of biofilm development in vitro. Results Candida biofilms could be visualized by SEM directly from denture samples from patients with denture stomatitis. These biofilms showed a propensity to adhere along cracks and imperfections of the denture acrylic. C albicans clinical isolates were able to form biofilms in vitro, although differences in the extent of biofilm formation were observed for different isolates recovered from the same patient. Susceptibility testing indicated that the resulting biofilms showed increased resistance to antifungal treatment. Presence of serum and saliva conditioning films increased the initial adherence of selected isolates but had little effect in overall biofilm formation. Conclusions Candida biofilms play a role in denture stomatitis.
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U2 - 10.1016/j.tripleo.2003.04.002
DO - 10.1016/j.tripleo.2003.04.002
M3 - Article
C2 - 15243471
AN - SCOPUS:3242688659
SN - 1079-2104
VL - 98
SP - 53
EP - 59
JO - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
JF - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology
IS - 1
ER -