Dental methacrylates may exert genotoxic effects via the oxidative induction of DNA double strand breaks and the inhibition of their repair

Janusz Blasiak, Ewelina Synowiec, Justyna Tarnawska, Piotr Czarny, Tomasz Poplawski, Russel J. Reiter

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Methacrylate monomers used in dentistry have been shown to induce DNA double strand breaks (DSBs), one of the most serious DNA damage. In the present work we show that a model dental adhesive consisting of 45% 2-hydroxyethyl methacrylate (HEMA) and 55% bisphenol A-diglycidyl dimethacrylate (Bis-GMA) at concentrations up to 0.25 mM Bis-GMA induced oxidative DNA in cultured primary human gingival fibroblasts (HGFs) as evaluated by the comet assay and probed with human 8-hydroxyguanine DNA-glycosylase 1. HEMA/Bis-GMA induced DSBs in HGFs as assessed by the neutral comet assay and phosphorylation of the H2AX histone and sodium ascorbate or melatonin (5-methoxy-N-acetyltryp-tamine) both at 50 μM reduced the DSBs, they also inhibited apoptosis induced by HEMA/Bis-GMA. The adhesive slowed the kinetics of the repair of DNA damage induced by hydrogen peroxide in HGFs, while sodium ascorbate or melatonin improved the efficacy of H2O2-induced damage in the presence of the methacrylates. The adhesive induced a rise in the G2/M cell population, accompanied by a reduction in the S cell population and an increase in G0/G1 cell population. Sodium ascorbate or melatonin elevated the S population and reduced the G2/M population. In conclusion, HEMA/Bis-GMA induce DSBs through, at least in part, oxidative mechanisms, and these compounds may interfere with DSBs repair. Vitamin C or melatonin may reduce the detrimental effects induced by methacrylates applied in dentistry.

Original languageEnglish (US)
Pages (from-to)7487-7496
Number of pages10
JournalMolecular Biology Reports
Volume39
Issue number7
DOIs
StatePublished - Jul 2012

Keywords

  • 2-hydroxyethyl methacrylate
  • Bis-GMA
  • Bisphenol A-diglycidyl dimethacrylate
  • DNA damage
  • DNA double-strand breaks
  • DNA repair
  • HEMA
  • Melatonin
  • Methacrylate-based dental materials
  • Vitamin C

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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