Denervation induces cytosolic phospholipase a 2-mediated fatty acid hydroperoxide generation by muscle mitochondria

Arunabh Bhattacharya, Florian L. Muller, Yuhong Liu, Marian Sabia, Hanyu Liang, Wook Song, Youngmok C. Jang, Qitao Ran, Holly Van Remmen

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


Previously, we demonstrated that mitochondria from dener- vated muscle exhibited dramatically higher Amplex Red dependent fluorescence (thought to be highly specific for hydrogen peroxide) compared with control muscle mitochondria. We now demonstrate that catalase only partially inhibits the Amplex Red signal in mitochondria from denervated muscle. In contrast, ebselen (a glutathione peroxidase mimetic and inhibitor of fatty acid hydroperoxides) significantly inhibits the Amplex Red signal. This suggests that the majority of the Amplex Red signal in mitochondria from denervated muscle is not derived from hydrogen peroxide. Because Amplex Red cannot react with substrates in the lipid environment, we hypothesize that lipid hydroperoxides formed within the mitochondrial lipid bilayer are released as fatty acid hydroperoxides and react with the Amplex Red probe. We also suggest that the release of fatty acid hydroperoxides from dener- vated muscle mitochondria may be an important determinant of muscle atrophy. In support of this, muscle atrophy and the Amplex Red signal are inhibited in caloric restricted mice and in transgenic mice that overexpress the lipid hydroperoxide-detoxifying enzyme glutathione peroxidase 4. Finally, we propose that cytosolic phospholipase A 2 may be a potential source of these hydroperoxides.

Original languageEnglish (US)
Pages (from-to)46-55
Number of pages10
JournalJournal of Biological Chemistry
Issue number1
StatePublished - Jan 2 2009

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Denervation induces cytosolic phospholipase a <sub>2</sub>-mediated fatty acid hydroperoxide generation by muscle mitochondria'. Together they form a unique fingerprint.

Cite this