Abstract
-Casein is chemotactic for human neutrophils (PMNs) and monocytes. The binding of fluorescein (FITC) -conjugated casein (mixture of α, β, and κ- casein) and purified α-casein to PMNs, monocytes, and lymphocytes was analyzed using flow cytometry. These studies demonstrate that 75-95% of PMNs and 46-85% of monocytes have membrane receptors for casein while lymphocytes lack these receptors. The binding of FITC-casein and FITC-α-casein was specific and was blocked only by unlabeled casein and α-casein, but not by ovalbumin, bovine or human serum albumin, β-casomorphin, C5a, or formyl-methionyl-leucylphenylalanine (fMLP). The binding of FITC-casein was reversible when PMNs were stained with this fluorescent agent and subsequently incubated with unlabeled casein. Double-labeling studies of mononuclear cells using FITC-casein and the OKM1 monoclonal antibody in conjunction with a rhodamine conjugated anti-Ig second antibody demonstrate that mononuclear cells binding FITC-casein also stain with the OKM1 monoclonal antibody, indicating a specificity for monocytes.
Original language | English (US) |
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Pages (from-to) | 363-375 |
Number of pages | 13 |
Journal | Inflammation |
Volume | 7 |
Issue number | 4 |
DOIs | |
State | Published - Dec 1983 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology