Demonstration of specific receptors for fluoresceinated casein on human neutrophils and monocytes using flow cytometry

Sharon L. Lewis, Dennis E. Van Epps

    Research output: Contribution to journalArticle

    18 Scopus citations

    Abstract

    -Casein is chemotactic for human neutrophils (PMNs) and monocytes. The binding of fluorescein (FITC) -conjugated casein (mixture of α, β, and κ- casein) and purified α-casein to PMNs, monocytes, and lymphocytes was analyzed using flow cytometry. These studies demonstrate that 75-95% of PMNs and 46-85% of monocytes have membrane receptors for casein while lymphocytes lack these receptors. The binding of FITC-casein and FITC-α-casein was specific and was blocked only by unlabeled casein and α-casein, but not by ovalbumin, bovine or human serum albumin, β-casomorphin, C5a, or formyl-methionyl-leucylphenylalanine (fMLP). The binding of FITC-casein was reversible when PMNs were stained with this fluorescent agent and subsequently incubated with unlabeled casein. Double-labeling studies of mononuclear cells using FITC-casein and the OKM1 monoclonal antibody in conjunction with a rhodamine conjugated anti-Ig second antibody demonstrate that mononuclear cells binding FITC-casein also stain with the OKM1 monoclonal antibody, indicating a specificity for monocytes.

    Original languageEnglish (US)
    Pages (from-to)363-375
    Number of pages13
    JournalInflammation
    Volume7
    Issue number4
    DOIs
    StatePublished - Dec 1983

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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