DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS

J. R. Wands; R. A. Marciniak; K. J. Isselbacher; M. Varghese; G. Don; J. W. Halliday; L. W. Powell

doi:10.1016/S0140-6736(82)91988-2

DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS

J. R. Wands, R. A. Marciniak, K. J. Isselbacher, M. Varghese, G. Don, J. W. Halliday, L. W. Powell

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

High-affinity IgM and IgG monoclonal antibodies (anti-HBs) against hepatitis B surface antigen (HBsAg) determinants were used to study a confined Australian Aboriginal population, 51% of which showed evidence of exposure to hepatitis B virus (HBV). A conventional radioimmunoassay which uses polyvalent anti-HBs antisera indicated that 4.4% ofthe subjects were positive for HBsAg; a monoclonal IgM anti-HBs radioimmunoassay detected all these HBsAg-positive samples and showed enhanced binding activity in a further 5·4% of subjects. Detailed analysis of this binding activity in serum by different IgG and IgM monoclonal anti-HBs antibodies demonstrated additional HBsAg-associated determinants as well as remarkable homogeneity of the determinants in this population. It was concluded that there are HBV or HBV-like agents in this community not previously detected by conventional assays.

Original language	English (US)
Pages (from-to)	977-980
Number of pages	4
Journal	The Lancet
Volume	319
Issue number	8279
DOIs	https://doi.org/10.1016/S0140-6736(82)91988-2
State	Published - May 1 1982
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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10.1016/S0140-6736(82)91988-2

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DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS. / Wands, J. R.; Marciniak, R. A.; Isselbacher, K. J.; Varghese, M.; Don, G.; Halliday, J. W.; Powell, L. W.

In: The Lancet, Vol. 319, No. 8279, 01.05.1982, p. 977-980.

Research output: Contribution to journal › Article › peer-review

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title = "DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS",

abstract = "High-affinity IgM and IgG monoclonal antibodies (anti-HBs) against hepatitis B surface antigen (HBsAg) determinants were used to study a confined Australian Aboriginal population, 51% of which showed evidence of exposure to hepatitis B virus (HBV). A conventional radioimmunoassay which uses polyvalent anti-HBs antisera indicated that 4.4% ofthe subjects were positive for HBsAg; a monoclonal IgM anti-HBs radioimmunoassay detected all these HBsAg-positive samples and showed enhanced binding activity in a further 5·4% of subjects. Detailed analysis of this binding activity in serum by different IgG and IgM monoclonal anti-HBs antibodies demonstrated additional HBsAg-associated determinants as well as remarkable homogeneity of the determinants in this population. It was concluded that there are HBV or HBV-like agents in this community not previously detected by conventional assays.",

author = "Wands, {J. R.} and Marciniak, {R. A.} and Isselbacher, {K. J.} and M. Varghese and G. Don and Halliday, {J. W.} and Powell, {L. W.}",

note = "Funding Information: We thank N. R. Brown for excellent technical assistance and Dr C. Allardyce of the Royal Flying Doctor Service, Queensland, Australia, for assistance in obtaining blood samples. This work was supported in part by grants AG-04154 J. R. W.) and by M. I. M. Holdings, Australia.and AA-02666 and 1 -K02-AA00048 from the National Institutes of Health (to",

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DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS

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