DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS

J. R. Wands; R. A. Marciniak; K. J. Isselbacher; M. Varghese; G. Don; J. W. Halliday; L. W. Powell

doi:10.1016/S0140-6736(82)91988-2

DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS

J. R. Wands, R. A. Marciniak, K. J. Isselbacher, M. Varghese, G. Don, J. W. Halliday, L. W. Powell

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

High-affinity IgM and IgG monoclonal antibodies (anti-HBs) against hepatitis B surface antigen (HBsAg) determinants were used to study a confined Australian Aboriginal population, 51% of which showed evidence of exposure to hepatitis B virus (HBV). A conventional radioimmunoassay which uses polyvalent anti-HBs antisera indicated that 4.4% ofthe subjects were positive for HBsAg; a monoclonal IgM anti-HBs radioimmunoassay detected all these HBsAg-positive samples and showed enhanced binding activity in a further 5·4% of subjects. Detailed analysis of this binding activity in serum by different IgG and IgM monoclonal anti-HBs antibodies demonstrated additional HBsAg-associated determinants as well as remarkable homogeneity of the determinants in this population. It was concluded that there are HBV or HBV-like agents in this community not previously detected by conventional assays.

Original language	English (US)
Pages (from-to)	977-980
Number of pages	4
Journal	The Lancet
Volume	319
Issue number	8279
DOIs	https://doi.org/10.1016/S0140-6736(82)91988-2
State	Published - May 1 1982
Externally published	Yes

ASJC Scopus subject areas

Medicine(all)

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DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS. / Wands, J. R.; Marciniak, R. A.; Isselbacher, K. J.; Varghese, M.; Don, G.; Halliday, J. W.; Powell, L. W.

In: The Lancet, Vol. 319, No. 8279, 01.05.1982, p. 977-980.

Research output: Contribution to journal › Article › peer-review

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title = "DEMONSTRATION OF PREVIOUSLY UNDETECTED HEPATITIS B VIRAL DETERMINANTS IN AN AUSTRALIAN ABORIGINAL POPULATION BY MONOCLONAL ANTI-HBs ANTIBODY RADIOIMMUNOASSAYS",

abstract = "High-affinity IgM and IgG monoclonal antibodies (anti-HBs) against hepatitis B surface antigen (HBsAg) determinants were used to study a confined Australian Aboriginal population, 51% of which showed evidence of exposure to hepatitis B virus (HBV). A conventional radioimmunoassay which uses polyvalent anti-HBs antisera indicated that 4.4% ofthe subjects were positive for HBsAg; a monoclonal IgM anti-HBs radioimmunoassay detected all these HBsAg-positive samples and showed enhanced binding activity in a further 5·4% of subjects. Detailed analysis of this binding activity in serum by different IgG and IgM monoclonal anti-HBs antibodies demonstrated additional HBsAg-associated determinants as well as remarkable homogeneity of the determinants in this population. It was concluded that there are HBV or HBV-like agents in this community not previously detected by conventional assays.",

author = "Wands, {J. R.} and Marciniak, {R. A.} and Isselbacher, {K. J.} and M. Varghese and G. Don and Halliday, {J. W.} and Powell, {L. W.}",

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