Demonstration by a rosette-forming technique of surface receptors for low-density lipoproteins on lymphoblastoid cells

A. J. Valente, C. J. Morris, K. W. Walton

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A simple technique of 'rosette' formation by low-density-lipoprotein-coated erythrocytes around human lymphoblastoid cells, stimulated to generate specific high affinity low-density lipoprotein receptors, is described. Rosette formation is specific for high-affinity surface binding alone and the properties of the low-density lipoprotein receptors defined by it are identical with those found by using 125I-labelled low-density lipoprotein binding at 4°C. Preliminary observations suggest that specific surface binding of low-density lipoprotein is shown by some, but not all, lymphoid cell lines, is independent of certain other surface receptors and is unrelated to whether the cells originate from B or T cells. Loss of low-density lipoprotein surface receptors on lymphoid cell lines (as with other surface receptors) may occur simply from prolonged culture rather than as a result of genetically mediated familial abnormality.

Original languageEnglish (US)
Pages (from-to)265-273
Number of pages9
JournalClinical science
Volume59
Issue number4
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • Medicine(all)

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