Deletion of the autoregulatory insert modulates intraprotein electron transfer in rat neuronal nitric oxide synthase

Changjian Feng, Linda J. Roman, James T. Hazzard, Dipak K. Ghosh, Gordon Tollin, Bettie Sue S. Masters

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27 Scopus citations

Abstract

Comparative CO photolysis kinetics studies on wild-type and autoregulatory (AR) insert-deletion mutant of rat nNOS holoenzyme were conducted to directly investigate the role of the unique AR insert in the catalytically significant FMN-heme intraprotein electron transfer (IET). Although the amplitude of the IET kinetic traces was decreased two- to three-fold, the AR deletion did not change the rate constant for the calmodulin-controlled IET. This suggests that the rate-limiting conversion of the electron-accepting state to a new electron-donating (output) state does not involve interactions with the AR insert, but that AR may stabilize the output state once it is formed.

Original languageEnglish (US)
Pages (from-to)2768-2772
Number of pages5
JournalFEBS Letters
Volume582
Issue number18
DOIs
Publication statusPublished - Aug 6 2008

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Keywords

  • Electron transfer
  • Kinetic
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Feng, C., Roman, L. J., Hazzard, J. T., Ghosh, D. K., Tollin, G., & Masters, B. S. S. (2008). Deletion of the autoregulatory insert modulates intraprotein electron transfer in rat neuronal nitric oxide synthase. FEBS Letters, 582(18), 2768-2772. https://doi.org/10.1016/j.febslet.2008.07.005