Deletion of the autoregulatory insert modulates intraprotein electron transfer in rat neuronal nitric oxide synthase

Changjian Feng, Linda J. Roman, James T. Hazzard, Dipak K. Ghosh, Gordon Tollin, Bettie Sue S. Masters

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Comparative CO photolysis kinetics studies on wild-type and autoregulatory (AR) insert-deletion mutant of rat nNOS holoenzyme were conducted to directly investigate the role of the unique AR insert in the catalytically significant FMN-heme intraprotein electron transfer (IET). Although the amplitude of the IET kinetic traces was decreased two- to three-fold, the AR deletion did not change the rate constant for the calmodulin-controlled IET. This suggests that the rate-limiting conversion of the electron-accepting state to a new electron-donating (output) state does not involve interactions with the AR insert, but that AR may stabilize the output state once it is formed.

Original languageEnglish (US)
Pages (from-to)2768-2772
Number of pages5
JournalFEBS Letters
Volume582
Issue number18
DOIs
StatePublished - Aug 6 2008

Keywords

  • Electron transfer
  • Kinetic
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Deletion of the autoregulatory insert modulates intraprotein electron transfer in rat neuronal nitric oxide synthase'. Together they form a unique fingerprint.

Cite this