Deletion mapping of HLA and chromosome 6p genes

F. Levine, H. Erlich, B. Mach, R. Leach, R. White, D. Pious

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

We have analyzed a set of heterozygous mutants with deletions that encompass parts of HLA and surrounding regions of chromosome 6p. By a combination of Southern blotting, serologic, enzymatic, and cytogenetic analyses, we have ordered eight independent deletion break points into a sequence that divides chromosome 6p into six regions. The deletion mutants have been used in conjunction with the Southern blot technique to map HLA and other 6p gene probes into those regions. On the basis of these and other data we propose a genetic and physical map of HLA and surrounding regions of chromosome 6p. We find that for HLA probes, most of which hybridize with more than one gene, the multiple copies recognized by single probes map to single regions. Any chromosome 6p gene can now be regionally mapped by using these mutants.

Original languageEnglish (US)
Pages (from-to)3741-3745
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number11
DOIs
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • General

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