Definition of T cell idiotypes using anti-idiotypic antisera produced by immunization with T cell clones

A. J. Infante, P. D. Infante, S. Gillis, C. G. Fathman

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Alloreactive T cell clones with distinct specificities were used to raise anti-idiotypic antisera via an F2 anti-(parent anti-F1) protocol. Antisera were raised that could stimulate the proliferation of the appropriate T cell clone, but not other clones. The active fraction of the antisera for T cell proliferation was immunoglobulin. In addition to proliferation, an anti-idiotypic antiserum could induce the appropriate T cell clone to secrete substantial amounts of interleukin 2 (IL-2). Production of IL-2 appeared independent of the involvement of accessory cells. These accessory cells may be unneccassary for IL-2 production in our assay, or their effect may be produced by anti-idiotype. Thus, anti-idiotype may provide two or more specific T cell signals.

Original languageEnglish (US)
Pages (from-to)1100-1107
Number of pages8
JournalJournal of Experimental Medicine
Volume155
Issue number4
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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