Defective immune function of primary effusion lymphoma cells is associated with distinct KSHV gene expression profiles

Todd J. Suscovich, Mini Paulose-Murphy, Jason D. Harlow, Yidong Chen, Seddon Y. Thomas, Tiffany J. Mellott, Bruce D. Walker, David T. Scadden, Steven Zeichner, Christian Brander

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Primary effusion lymphomas (PEL) are uniformly infected with Kaposi's sarcoma-associated herpesvirus (KSHV), and thus likely present both tumor and viral antigens to the immune system. In order to grow unrestricted and cause disease, multiple immune evasion strategies may be utilized by PEL to evade immune surveillance. Using six well-established PEL cell lines and comparing these to Epstein - Barr virus-transformed B cell lines and peripheral blood B cells, significant differences were found in the surface expression of molecules involved in antigen presentation, T cell activation and cell - cell adhesion. Significantly reduced stimulation of cytotoxic T lymphocytes, lowered sensitivity to natural killer cell-mediated lysis and impaired function as antigen presenting cells in mixed leukocyte reactions were found for three PEL cell lines with particularly low CD54, CD58 and CD81 expression. Comparative microarray analysis demonstrated specific patterns of KSHV-encoded gene expression that were associated with the different immune functions of these cell lines. Thus, the present data suggest that distinct patterns of KSHV gene expression may be associated with particular phenotypic and functional characteristics of PEL cells, which may influence PEL pathogenesis.

Original languageEnglish (US)
Pages (from-to)1223-1238
Number of pages16
JournalLeukemia and Lymphoma
Volume45
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Primary Effusion Lymphoma
Human Herpesvirus 8
Transcriptome
Cell Line
B-Lymphocytes
Immune Evasion
Gene Expression
Transformed Cell Line
Mixed Lymphocyte Culture Test
Viral Tumor Antigens
Antigen Presentation
Cytotoxic T-Lymphocytes
Antigen-Presenting Cells
Microarray Analysis
Human Herpesvirus 4
Cell Adhesion
Natural Killer Cells
Immune System
Blood Cells
T-Lymphocytes

Keywords

  • Immune evasion
  • Immune phenotype
  • KSHV
  • PEL

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Suscovich, T. J., Paulose-Murphy, M., Harlow, J. D., Chen, Y., Thomas, S. Y., Mellott, T. J., ... Brander, C. (2004). Defective immune function of primary effusion lymphoma cells is associated with distinct KSHV gene expression profiles. Leukemia and Lymphoma, 45(6), 1223-1238. https://doi.org/10.1080/10428190310001643358

Defective immune function of primary effusion lymphoma cells is associated with distinct KSHV gene expression profiles. / Suscovich, Todd J.; Paulose-Murphy, Mini; Harlow, Jason D.; Chen, Yidong; Thomas, Seddon Y.; Mellott, Tiffany J.; Walker, Bruce D.; Scadden, David T.; Zeichner, Steven; Brander, Christian.

In: Leukemia and Lymphoma, Vol. 45, No. 6, 06.2004, p. 1223-1238.

Research output: Contribution to journalArticle

Suscovich, TJ, Paulose-Murphy, M, Harlow, JD, Chen, Y, Thomas, SY, Mellott, TJ, Walker, BD, Scadden, DT, Zeichner, S & Brander, C 2004, 'Defective immune function of primary effusion lymphoma cells is associated with distinct KSHV gene expression profiles', Leukemia and Lymphoma, vol. 45, no. 6, pp. 1223-1238. https://doi.org/10.1080/10428190310001643358
Suscovich, Todd J. ; Paulose-Murphy, Mini ; Harlow, Jason D. ; Chen, Yidong ; Thomas, Seddon Y. ; Mellott, Tiffany J. ; Walker, Bruce D. ; Scadden, David T. ; Zeichner, Steven ; Brander, Christian. / Defective immune function of primary effusion lymphoma cells is associated with distinct KSHV gene expression profiles. In: Leukemia and Lymphoma. 2004 ; Vol. 45, No. 6. pp. 1223-1238.
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