Deep multi-OMICs and multi-tissue characterization in a pre-and postprandial state in human volunteers

The GEMM family study research design

Raul A. Bastarrachea, Hugo A. Laviada-Molina, Edna J. Nava-Gonzalez, Irene Leal-Berumen, Claudia Escudero-Lourdes, Fabiola Escalante-Araiza, Vanessa Giselle Peschard, Rosa A. Veloz-Garza, Karin Haack, Angélica Martínez-Hernández, Francisco M. Barajas-Olmos, Fernanda Molina-Segui, Fatima A. Buenfil-Rello, Lucia Gonzalez-Ramirez, Reinhard Janssen-Aguilar, Ricardo Lopez-Muñoz, Fernanda Perez-Cetina, Janeth F. Gaytan-Saucedo, Zoila Vaquera, Judith Cornejo-Barrera & 19 others Juan Carlos Castillo-Pineda, Areli Murillo-Ramirez, Sara P. Diaz-Tena, Benigno Figueroa-Nuñez, Laura González-López, Rocío A. Salinas-Osornio, Melesio E. Valencia-Rendón, José Ángeles-Chimal, Jesús Santa Olalla Tapia, José M. Remes-Troche, Salvador B. Valdovinos-Chavez, Eira E. Huerta-Avila, Xianlin Han, Lorena Orozco, Ernesto Rodriguez-Ayala, Susan E Weintraub, Esther C. Gallegos-Cabrales, Shelley A. Cole, Jack W. Kent

Research output: Contribution to journalArticle

Abstract

Cardiovascular disease (CVD) and type 2 diabetes (T2D) are increasing worldwide. This is mainly due to an unhealthy nutrition, implying that variation in CVD risk may be due to variation in the capacity to manage a nutritional load. We examined the genomic basis of postprandial metabolism. Our main purpose was to introduce the GEMM Family Study (Genetics of Metabolic Diseases in Mexico) as a multi-center study carrying out an ongoing recruitment of healthy urban adults. Each participant received a mixed meal challenge and provided a 5-hours’ time course series of blood, buffy coat specimens for DNA isolation, and adipose tissue (ADT)/skeletal muscle (SKM) biopsies at fasting and 3 h after the meal. A comprehensive profiling, including metabolomic signatures in blood and transcriptomic and proteomic profiling in SKM and ADT, was performed to describe tendencies for variation in postprandial response. Our data generation methods showed preliminary trends indicating that by characterizing the dynamic properties of biomarkers with metabolic activity and analyzing multi-OMICS data it could be possible, with this methodology and research design, to identify early trends for molecular biology systems and genes involved in the fasted and fed states.

Original languageEnglish (US)
Article number532
JournalGenes
Volume9
Issue number11
DOIs
StatePublished - Nov 2 2018

Fingerprint

Meals
Adipose Tissue
Volunteers
Skeletal Muscle
Blood Buffy Coat
Research Design
Cardiovascular Diseases
Inborn Genetic Diseases
Metabolomics
Metabolic Diseases
Mexico
Proteomics
Type 2 Diabetes Mellitus
Molecular Biology
Fasting
Biomarkers
Biopsy
Muscles
DNA
Genes

Keywords

  • Gemm family study
  • Mixed meal challenge
  • Multi-OMICs
  • Postprandial metabolism

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Bastarrachea, R. A., Laviada-Molina, H. A., Nava-Gonzalez, E. J., Leal-Berumen, I., Escudero-Lourdes, C., Escalante-Araiza, F., ... Kent, J. W. (2018). Deep multi-OMICs and multi-tissue characterization in a pre-and postprandial state in human volunteers: The GEMM family study research design. Genes, 9(11), [532]. https://doi.org/10.3390/genes9110532

Deep multi-OMICs and multi-tissue characterization in a pre-and postprandial state in human volunteers : The GEMM family study research design. / Bastarrachea, Raul A.; Laviada-Molina, Hugo A.; Nava-Gonzalez, Edna J.; Leal-Berumen, Irene; Escudero-Lourdes, Claudia; Escalante-Araiza, Fabiola; Peschard, Vanessa Giselle; Veloz-Garza, Rosa A.; Haack, Karin; Martínez-Hernández, Angélica; Barajas-Olmos, Francisco M.; Molina-Segui, Fernanda; Buenfil-Rello, Fatima A.; Gonzalez-Ramirez, Lucia; Janssen-Aguilar, Reinhard; Lopez-Muñoz, Ricardo; Perez-Cetina, Fernanda; Gaytan-Saucedo, Janeth F.; Vaquera, Zoila; Cornejo-Barrera, Judith; Castillo-Pineda, Juan Carlos; Murillo-Ramirez, Areli; Diaz-Tena, Sara P.; Figueroa-Nuñez, Benigno; González-López, Laura; Salinas-Osornio, Rocío A.; Valencia-Rendón, Melesio E.; Ángeles-Chimal, José; Tapia, Jesús Santa Olalla; Remes-Troche, José M.; Valdovinos-Chavez, Salvador B.; Huerta-Avila, Eira E.; Han, Xianlin; Orozco, Lorena; Rodriguez-Ayala, Ernesto; Weintraub, Susan E; Gallegos-Cabrales, Esther C.; Cole, Shelley A.; Kent, Jack W.

In: Genes, Vol. 9, No. 11, 532, 02.11.2018.

Research output: Contribution to journalArticle

Bastarrachea, RA, Laviada-Molina, HA, Nava-Gonzalez, EJ, Leal-Berumen, I, Escudero-Lourdes, C, Escalante-Araiza, F, Peschard, VG, Veloz-Garza, RA, Haack, K, Martínez-Hernández, A, Barajas-Olmos, FM, Molina-Segui, F, Buenfil-Rello, FA, Gonzalez-Ramirez, L, Janssen-Aguilar, R, Lopez-Muñoz, R, Perez-Cetina, F, Gaytan-Saucedo, JF, Vaquera, Z, Cornejo-Barrera, J, Castillo-Pineda, JC, Murillo-Ramirez, A, Diaz-Tena, SP, Figueroa-Nuñez, B, González-López, L, Salinas-Osornio, RA, Valencia-Rendón, ME, Ángeles-Chimal, J, Tapia, JSO, Remes-Troche, JM, Valdovinos-Chavez, SB, Huerta-Avila, EE, Han, X, Orozco, L, Rodriguez-Ayala, E, Weintraub, SE, Gallegos-Cabrales, EC, Cole, SA & Kent, JW 2018, 'Deep multi-OMICs and multi-tissue characterization in a pre-and postprandial state in human volunteers: The GEMM family study research design', Genes, vol. 9, no. 11, 532. https://doi.org/10.3390/genes9110532
Bastarrachea RA, Laviada-Molina HA, Nava-Gonzalez EJ, Leal-Berumen I, Escudero-Lourdes C, Escalante-Araiza F et al. Deep multi-OMICs and multi-tissue characterization in a pre-and postprandial state in human volunteers: The GEMM family study research design. Genes. 2018 Nov 2;9(11). 532. https://doi.org/10.3390/genes9110532
Bastarrachea, Raul A. ; Laviada-Molina, Hugo A. ; Nava-Gonzalez, Edna J. ; Leal-Berumen, Irene ; Escudero-Lourdes, Claudia ; Escalante-Araiza, Fabiola ; Peschard, Vanessa Giselle ; Veloz-Garza, Rosa A. ; Haack, Karin ; Martínez-Hernández, Angélica ; Barajas-Olmos, Francisco M. ; Molina-Segui, Fernanda ; Buenfil-Rello, Fatima A. ; Gonzalez-Ramirez, Lucia ; Janssen-Aguilar, Reinhard ; Lopez-Muñoz, Ricardo ; Perez-Cetina, Fernanda ; Gaytan-Saucedo, Janeth F. ; Vaquera, Zoila ; Cornejo-Barrera, Judith ; Castillo-Pineda, Juan Carlos ; Murillo-Ramirez, Areli ; Diaz-Tena, Sara P. ; Figueroa-Nuñez, Benigno ; González-López, Laura ; Salinas-Osornio, Rocío A. ; Valencia-Rendón, Melesio E. ; Ángeles-Chimal, José ; Tapia, Jesús Santa Olalla ; Remes-Troche, José M. ; Valdovinos-Chavez, Salvador B. ; Huerta-Avila, Eira E. ; Han, Xianlin ; Orozco, Lorena ; Rodriguez-Ayala, Ernesto ; Weintraub, Susan E ; Gallegos-Cabrales, Esther C. ; Cole, Shelley A. ; Kent, Jack W. / Deep multi-OMICs and multi-tissue characterization in a pre-and postprandial state in human volunteers : The GEMM family study research design. In: Genes. 2018 ; Vol. 9, No. 11.
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AU - Nava-Gonzalez, Edna J.

AU - Leal-Berumen, Irene

AU - Escudero-Lourdes, Claudia

AU - Escalante-Araiza, Fabiola

AU - Peschard, Vanessa Giselle

AU - Veloz-Garza, Rosa A.

AU - Haack, Karin

AU - Martínez-Hernández, Angélica

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AU - Molina-Segui, Fernanda

AU - Buenfil-Rello, Fatima A.

AU - Gonzalez-Ramirez, Lucia

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AU - Lopez-Muñoz, Ricardo

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AU - Gaytan-Saucedo, Janeth F.

AU - Vaquera, Zoila

AU - Cornejo-Barrera, Judith

AU - Castillo-Pineda, Juan Carlos

AU - Murillo-Ramirez, Areli

AU - Diaz-Tena, Sara P.

AU - Figueroa-Nuñez, Benigno

AU - González-López, Laura

AU - Salinas-Osornio, Rocío A.

AU - Valencia-Rendón, Melesio E.

AU - Ángeles-Chimal, José

AU - Tapia, Jesús Santa Olalla

AU - Remes-Troche, José M.

AU - Valdovinos-Chavez, Salvador B.

AU - Huerta-Avila, Eira E.

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