Deep multi-OMICs and multi-tissue characterization in a pre-and postprandial state in human volunteers: The GEMM family study research design

Raul A. Bastarrachea, Hugo A. Laviada-Molina, Edna J. Nava-Gonzalez, Irene Leal-Berumen, Claudia Escudero-Lourdes, Fabiola Escalante-Araiza, Vanessa Giselle Peschard, Rosa A. Veloz-Garza, Karin Haack, Angélica Martínez-Hernández, Francisco M. Barajas-Olmos, Fernanda Molina-Segui, Fatima A. Buenfil-Rello, Lucia Gonzalez-Ramirez, Reinhard Janssen-Aguilar, Ricardo Lopez-Muñoz, Fernanda Perez-Cetina, Janeth F. Gaytan-Saucedo, Zoila Vaquera, Judith Cornejo-BarreraJuan Carlos Castillo-Pineda, Areli Murillo-Ramirez, Sara P. Diaz-Tena, Benigno Figueroa-Nuñez, Laura González-López, Rocío A. Salinas-Osornio, Melesio E. Valencia-Rendón, José Ángeles-Chimal, Jesús Santa Olalla Tapia, José M. Remes-Troche, Salvador B. Valdovinos-Chavez, Eira E. Huerta-Avila, Xianlin Han, Lorena Orozco, Ernesto Rodriguez-Ayala, Susan Weintraub, Esther C. Gallegos-Cabrales, Shelley A. Cole, Jack W. Kent

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Cardiovascular disease (CVD) and type 2 diabetes (T2D) are increasing worldwide. This is mainly due to an unhealthy nutrition, implying that variation in CVD risk may be due to variation in the capacity to manage a nutritional load. We examined the genomic basis of postprandial metabolism. Our main purpose was to introduce the GEMM Family Study (Genetics of Metabolic Diseases in Mexico) as a multi-center study carrying out an ongoing recruitment of healthy urban adults. Each participant received a mixed meal challenge and provided a 5-hours’ time course series of blood, buffy coat specimens for DNA isolation, and adipose tissue (ADT)/skeletal muscle (SKM) biopsies at fasting and 3 h after the meal. A comprehensive profiling, including metabolomic signatures in blood and transcriptomic and proteomic profiling in SKM and ADT, was performed to describe tendencies for variation in postprandial response. Our data generation methods showed preliminary trends indicating that by characterizing the dynamic properties of biomarkers with metabolic activity and analyzing multi-OMICS data it could be possible, with this methodology and research design, to identify early trends for molecular biology systems and genes involved in the fasted and fed states.

Original languageEnglish (US)
Article number532
JournalGenes
Volume9
Issue number11
DOIs
StatePublished - Nov 2 2018

Keywords

  • Gemm family study
  • Mixed meal challenge
  • Multi-OMICs
  • Postprandial metabolism

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Bastarrachea, R. A., Laviada-Molina, H. A., Nava-Gonzalez, E. J., Leal-Berumen, I., Escudero-Lourdes, C., Escalante-Araiza, F., Peschard, V. G., Veloz-Garza, R. A., Haack, K., Martínez-Hernández, A., Barajas-Olmos, F. M., Molina-Segui, F., Buenfil-Rello, F. A., Gonzalez-Ramirez, L., Janssen-Aguilar, R., Lopez-Muñoz, R., Perez-Cetina, F., Gaytan-Saucedo, J. F., Vaquera, Z., ... Kent, J. W. (2018). Deep multi-OMICs and multi-tissue characterization in a pre-and postprandial state in human volunteers: The GEMM family study research design. Genes, 9(11), [532]. https://doi.org/10.3390/genes9110532