Deconstructing Pancreas Development to Reconstruct Human Islets from Pluripotent Stem Cells

Kristen D. McKnight; Pei Wang; Seung K. Kim

doi:10.1016/j.stem.2010.03.003

Deconstructing Pancreas Development to Reconstruct Human Islets from Pluripotent Stem Cells

Kristen D. McKnight, Pei Wang, Seung K. Kim

Research output: Contribution to journal › Review article › peer-review

48 Scopus citations

Abstract

There is considerable excitement about harnessing the potential of human stem cells to replace pancreatic islets that are destroyed in type 1 diabetes mellitus. However, our current understanding of the mechanisms underlying pancreas and islet ontogeny has come largely from the powerful genetic, developmental, and embryological approaches available in nonhuman organisms. Successful islet reconstruction from human pluripotent cells will require greater attention to "deconstructing" human pancreas and islet developmental biology and consistent application of conditional genetics, lineage tracing, and cell purification to stem cell biology.

Original language	English (US)
Pages (from-to)	300-308
Number of pages	9
Journal	Cell Stem Cell
Volume	6
Issue number	4
DOIs	https://doi.org/10.1016/j.stem.2010.03.003
State	Published - Apr 2 2010
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

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10.1016/j.stem.2010.03.003

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abstract = "There is considerable excitement about harnessing the potential of human stem cells to replace pancreatic islets that are destroyed in type 1 diabetes mellitus. However, our current understanding of the mechanisms underlying pancreas and islet ontogeny has come largely from the powerful genetic, developmental, and embryological approaches available in nonhuman organisms. Successful islet reconstruction from human pluripotent cells will require greater attention to {"}deconstructing{"} human pancreas and islet developmental biology and consistent application of conditional genetics, lineage tracing, and cell purification to stem cell biology.",

author = "McKnight, {Kristen D.} and Pei Wang and Kim, {Seung K.}",

note = "Funding Information: We thank members of the S.K.K. group for advice and assistance. K.D.M. is supported by a Juvenile Diabetes Research Foundation (JDRF) fellowship and P.W. was supported by a Larry L. Hillblom Foundation (LLHF) fellowship. The S.K.K. group was supported by the Mead Foundation, Snyder Foundation, Stinehart Foundation, Harry and Leona Helmsley Foundation, JDRF, LLHF, California Institute of Regenerative Medicine, the U.S. National Institutes of Health, and the Howard Hughes Medical Institute (HHMI). S.K.K. is an Investigator of the HHMI. We apologize to colleagues whose work we could not cite because of space constraints.",

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doi = "10.1016/j.stem.2010.03.003",

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AU - Wang, Pei

AU - Kim, Seung K.

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PY - 2010/4/2

Y1 - 2010/4/2

N2 - There is considerable excitement about harnessing the potential of human stem cells to replace pancreatic islets that are destroyed in type 1 diabetes mellitus. However, our current understanding of the mechanisms underlying pancreas and islet ontogeny has come largely from the powerful genetic, developmental, and embryological approaches available in nonhuman organisms. Successful islet reconstruction from human pluripotent cells will require greater attention to "deconstructing" human pancreas and islet developmental biology and consistent application of conditional genetics, lineage tracing, and cell purification to stem cell biology.

AB - There is considerable excitement about harnessing the potential of human stem cells to replace pancreatic islets that are destroyed in type 1 diabetes mellitus. However, our current understanding of the mechanisms underlying pancreas and islet ontogeny has come largely from the powerful genetic, developmental, and embryological approaches available in nonhuman organisms. Successful islet reconstruction from human pluripotent cells will require greater attention to "deconstructing" human pancreas and islet developmental biology and consistent application of conditional genetics, lineage tracing, and cell purification to stem cell biology.

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Deconstructing Pancreas Development to Reconstruct Human Islets from Pluripotent Stem Cells

Abstract

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