DDAVP-induced maternal hyposmolality increases ovine fetal urine flow

M. J.M. Nijland, M. G. Ross, L. K. Kullama, K. Bradley, M. G. Ervin

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Fetal urine flow is influenced by fetal intravascular volume, glomerular filtration rate, tubular reabsorption, and fluid regulatory hormones. As maternal-to-fetal fluid transfer is dependent on hydrostatic and osmotic gradients, we postulated that a chronic decrease in maternal plasma osmolality would promote transplacental fluid transfer and increase fetal urine flow. Six pregnant ewes and singleton fetuses (131 ± 2 days; term = 150 days) received bladder and hindlimb arterial and venous catheters. After 5 days, plasma and urine composition, urine flow rate (U(vol)) and plasma arginine vasopressin (AVP) levels were measured during a 2-h control period. At 2 h, tap water (2 liter, 38°C) was administered to the ewe. At 3 h, ewes received a 20-μg bolus of 1-desamino-[D-Arg8]vasopressin (DDAVP), followed by continuous infusion (4 μg/h). In response to water loading, maternal urine osmolality decreased (761 ± 158 to 339 ± 13 mosmol/kgH2O), and U(vol) increased. After DDAVP, maternal urine osmolality increased (1,270 ± 89 mosmol/kgH2O), and U(vol), hematocrit, plasma osmolality (304 ± 1 to 284 ± 4 mosmol/kgH2O), and protein concentration decreased. Five hours after maternal DDAVP infusion, fetal plasma osmolality decreased (300 ± 1 to 281 ± 3 mosmol/kgH2O), and U(vol) increased (0.4 ± 0.1 to 1.3 ± 0.2 ml/min) and remained elevated at 24 h. There was no change in fetal plasma DDAVP (immunoreactive AVP) levels or fetal urine osmolality. Controlled changes in maternal plasma osmolality may prove useful in modulating fetal urine flow and, ultimately, amniotic fluid volume.

Original languageEnglish (US)
Pages (from-to)R358-R365
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Issue number2 37-2
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)


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