DBZ blocks Lps-induced monocyte activation and foam cell formation via inhibiting nuclear factor-κB

Xinni Xie, Shixiang Wang, Lei Xiao, Jun Zhang, Jing Wang, Jin Liu, Xuji Shen, Dacheng He, Xiaohui Zheng, Yonggong Zhai

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Background/Aims: It has been widely accepted that chronic inflammation plays important roles in the atherogenesis. Danshensu Bingpian Zhi (DBZ) is a novel synthetic compound derived from the traditional Chinese medicine (TCM) formula Fu Fang Dan Shen (FFDS), which is effective on atherosclerosis clinically. We hypothesized that DBZ possessed the anti-atherosclerosis potentials. Here, we examined the inhibitory effects of DBZ on LPS-induced monocyte activation and foam cell formation. Methods: The effects of DBZ were assessed on LPS-induced inflammatory factors expression in monocyte/macrophage. Activation of NF-κB and AP-1 was analyzed by luciferase reporter assay and signaling pathway of NF-κB was investigated to elucidate mechanisms underlying DBZ mediated anti-inflammatory activity. Effects of DBZ on macrophage lipid accumulation were evaluated in native LDL and LPS co-incubated macrophages. Results: DBZ inhibited LPS-induced inflammatory factors expression dose dependently in monocytes. DBZ inhibited NF-κB activation strongly and AP-1 slightly. DBZ suppressed the LPS-induced degradation of IκBα, thereby decreasing the translocation of p65 to nucleus. Furthermore, DBZ suppressed LPS-activated macrophages lipid accumulation, partly due to inhibiting the expression of LPS-induced aP2 and ADRP in macrophges. Conclusion: These results demonstrate that DBZ has potentials on anti-atherosclerosis by suppressing monocyte activation and foam cell formation.

Original languageEnglish (US)
Pages (from-to)649-662
Number of pages14
JournalCellular Physiology and Biochemistry
Volume28
Issue number4
DOIs
StatePublished - 2011
Externally publishedYes

Keywords

  • Foam cell
  • Inflammation
  • Monocyte activation
  • NF-κB

ASJC Scopus subject areas

  • Physiology

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