Cytotoxicity in cultured RPE: A comparative study between continuous wave and mode-locked lasers

Michael L. Denton; Debbie M. Eikum; David J. Stolarski; Gary D. Noojin; Robert J. Thomas; Randolph D. Glickman; Benjamin A. Rockwell

doi:10.1117/12.472519

Cytotoxicity in cultured RPE: A comparative study between continuous wave and mode-locked lasers

Michael L. Denton, Debbie M. Eikum, David J. Stolarski, Gary D. Noojin, Robert J. Thomas, Randolph D. Glickman, Benjamin A. Rockwell

Department of Ophthalmology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Scopus citations

Abstract

We have previously shown that minimum visible lesions (MVL) generated in vivo by exposures at threshold energies are not ophthalmoscopically visible at one hour, but become visible 24 hours post-exposure. Our preliminary studies toward understanding this phenomenon at the molecular level are presented. We provide threshold data relating cytotoxicity with energy for acute laser exposures in cultured retinal pigment epithelial cells. Rapid loss of cell viability is used as an initial endpoint of analysis. Comparisons of damage efficiency for continuous wave and mode-locked lasers are presented.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	S.L. Jacques, D.D. Duncan, S.J. Kirkpatrick, A. Kriete
Pages	156-160
Number of pages	5
Volume	4617
DOIs	https://doi.org/10.1117/12.472519
State	Published - 2002
Event	Laser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical - San Jose, CA, United States Duration: Jan 20 2002 → Jan 23 2002

Other

Other	Laser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical
Country/Territory	United States
City	San Jose, CA
Period	1/20/02 → 1/23/02

Keywords

Cytotoxicity
Fluorescence microscopy
Mode-locked
RPE cells

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

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10.1117/12.472519

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Denton, M. L., Eikum, D. M., Stolarski, D. J., Noojin, G. D., Thomas, R. J., Glickman, R. D., & Rockwell, B. A. (2002). Cytotoxicity in cultured RPE: A comparative study between continuous wave and mode-locked lasers. In S. L. Jacques, D. D. Duncan, S. J. Kirkpatrick, & A. Kriete (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4617, pp. 156-160) https://doi.org/10.1117/12.472519

Cytotoxicity in cultured RPE : A comparative study between continuous wave and mode-locked lasers. / Denton, Michael L.; Eikum, Debbie M.; Stolarski, David J. et al.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / S.L. Jacques; D.D. Duncan; S.J. Kirkpatrick; A. Kriete. Vol. 4617 2002. p. 156-160.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Denton, ML, Eikum, DM, Stolarski, DJ, Noojin, GD, Thomas, RJ, Glickman, RD & Rockwell, BA 2002, Cytotoxicity in cultured RPE: A comparative study between continuous wave and mode-locked lasers. in SL Jacques, DD Duncan, SJ Kirkpatrick & A Kriete (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4617, pp. 156-160, Laser Tissue Interaction XIII: Photochemical, Photothermal and Photomechanical, San Jose, CA, United States, 1/20/02. https://doi.org/10.1117/12.472519

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abstract = "We have previously shown that minimum visible lesions (MVL) generated in vivo by exposures at threshold energies are not ophthalmoscopically visible at one hour, but become visible 24 hours post-exposure. Our preliminary studies toward understanding this phenomenon at the molecular level are presented. We provide threshold data relating cytotoxicity with energy for acute laser exposures in cultured retinal pigment epithelial cells. Rapid loss of cell viability is used as an initial endpoint of analysis. Comparisons of damage efficiency for continuous wave and mode-locked lasers are presented.",

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AB - We have previously shown that minimum visible lesions (MVL) generated in vivo by exposures at threshold energies are not ophthalmoscopically visible at one hour, but become visible 24 hours post-exposure. Our preliminary studies toward understanding this phenomenon at the molecular level are presented. We provide threshold data relating cytotoxicity with energy for acute laser exposures in cultured retinal pigment epithelial cells. Rapid loss of cell viability is used as an initial endpoint of analysis. Comparisons of damage efficiency for continuous wave and mode-locked lasers are presented.

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Cytotoxicity in cultured RPE: A comparative study between continuous wave and mode-locked lasers

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