Cytotoxicity from tumor cells of cationic proteins from human neutrophil granules

Robert A Clark, I. Olsson, S. J. Klebanoff

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Abstract

Cationic proteins purified from human polymorphonuclear leukocyte granules (PMN) exert a cytotoxic effect on murine (Moloney virus induced) lymphoma cells in suspension cultures. This effect is time and concentration dependent, is inhibited by the anionic agent heparin, and is enhanced by preheating the cationic proteins. Other strongly basic proteins (histone, protamine) also exhibited cytotoxic activity. Myeloperoxidase (MPO) isolated from human leukocytes is cytotoxic when combined with H2O2 and chloride. Under these conditions, the potency of the myeloperoxidase-mediated system is greater than that of the cationic proteins. These studies extend earlier work on the cytotoxic effect of a system comprised of canine MPO, H2O2 and a halide, to human MPO. Thus, at least 2 bactericidal systems of the human PMN are capable of killing mammalian tumor cells. Although the myeloperoxidase system has been implicated in the cytotoxic effect of phagocytosing PMNs, further studies are required.

Original languageEnglish (US)
Pages (from-to)719-723
Number of pages5
JournalJournal of Cell Biology
Volume70
Issue number3
StatePublished - 1976
Externally publishedYes

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Peroxidase
Neoplasms
Proteins
Protamines
Phagocytosis
Histones
Heparin
Canidae
Chlorides
Lymphoma
Suspensions
Neutrophils
Leukocytes
neutrophil basic proteins
Viruses

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cytotoxicity from tumor cells of cationic proteins from human neutrophil granules. / Clark, Robert A; Olsson, I.; Klebanoff, S. J.

In: Journal of Cell Biology, Vol. 70, No. 3, 1976, p. 719-723.

Research output: Contribution to journalArticle

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