Cytokine regulation of cellular proliferation in endometriosis

N. A. Klein, G. M. Pergola, Rajeshwar R Tekmal, I. A. Montoya, T. D. Dey, Robert S Schenken

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Objective: This study was designed (1) to characterize the resident leukocyte population in ectopic endometrium (EE), (2) to assess proliferative activity of cellular components in EE, (3) to assess whether resident leukocytes in EE express IFNγ mRNA and (4) to demonstrate endometrial epithelial cell IFNγ receptors in EE. Study Design: Biopsies of EE and normal eutopic endometrium (UE) were studied immunocytochemically using monoclonal antibodies specific for CD45 leukocyte common antigen. CD3 (a T cell marker), CD 11 c (a macrophage marker), and Ki67 (proliferation marker). Leukocyte types were identified immunocytochemically, followed by in situ hybridization to assess expression of IFNγ mRNA. IFNγ receptor expression was assessed by immunocytochemistry. Results: The percentage of scattered stromal cells staining for each CD marker was greater in EE than in UE. The proliferative activity of endometrial stromal cells and epithelial cells was significantly less in EE than in UE. The overall concentration of T cells and macrophages expressing IFNγ mRNA was significantly greater in EE than in UE. The percentage of each leukocyte type expressing IFNγ mRNA was also greater in EE than in UE, and IFNγ receptors were present in glandular epithelium of EE. Conclusions: These findings support a possible paracrine role for resident leuko- cytes and IFNγ in regulating cell proliferation in endometriosis.

Original languageEnglish (US)
Pages (from-to)322-332
Number of pages11
JournalAnnals of the New York Academy of Sciences
Volume734
DOIs
StatePublished - 1994

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Endometriosis
Endometrium
Cell Proliferation
Cytokines
Messenger RNA
T-cells
Macrophages
Leukocytes
CD45 Antigens
Biopsy
Cell proliferation
Stromal Cells
Monoclonal Antibodies
Cells
Epithelial Cells
T-Lymphocytes
In Situ Hybridization
Epithelium
Immunohistochemistry
Staining and Labeling

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cytokine regulation of cellular proliferation in endometriosis. / Klein, N. A.; Pergola, G. M.; Tekmal, Rajeshwar R; Montoya, I. A.; Dey, T. D.; Schenken, Robert S.

In: Annals of the New York Academy of Sciences, Vol. 734, 1994, p. 322-332.

Research output: Contribution to journalArticle

Klein, N. A. ; Pergola, G. M. ; Tekmal, Rajeshwar R ; Montoya, I. A. ; Dey, T. D. ; Schenken, Robert S. / Cytokine regulation of cellular proliferation in endometriosis. In: Annals of the New York Academy of Sciences. 1994 ; Vol. 734. pp. 322-332.
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