Cytokine-induced expression of HIV-1 in a chronically infected promonocyte cell line

Thomas M. Folks, Jesse Justement, Audrey Kinter, Charles A. Dinarello, Anthony S. Fauci

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    Abstract

    A model system for cytokine-induced up-regulation of human immunodeficiency virus type 1 (HIV-1) expression in chronically infected promonocyte clones was established. The parent promonocyte cell line U937 was chronically infected with HIV-1 and from this line a clone, U1, was derived. U1 showed minimal constitutive expression of HIV-1, but virus expression was markedly up-regulated by a phytohemagglutinin-induced supernatant containing multiple cytokines and by recombinant granulocyte/macrophage colony-stimulating factor alone. Recombinant interleukin-1 (IL-1), IL-2, interferon-γ, and tumor necrosis factor-α did not up-regulate virus expression. Concomitant with the cytokine-induced up-regulation of HIV-1, expression of membrane-bound IL-1β was selectively induced in U1 in the absence of induction of other surface membrane proteins. This cytokine up-regulation of IL-1β was not seen in the uninfected parent U937 cell line. These studies have implications for the understanding of the mechanism of progression from a latent or low-level HIV-1 infection to a productive infection with resulting immunosuppression. In addition, this model can be used to delineate the potential mechanisms whereby HIV-1 infection regulates cellular gene expression.

    Original languageEnglish (US)
    Pages (from-to)800-802
    Number of pages3
    JournalScience
    Volume238
    Issue number4828
    DOIs
    StatePublished - Jan 1 1987

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    Folks, T. M., Justement, J., Kinter, A., Dinarello, C. A., & Fauci, A. S. (1987). Cytokine-induced expression of HIV-1 in a chronically infected promonocyte cell line. Science, 238(4828), 800-802. https://doi.org/10.1126/science.3313729