TY - JOUR
T1 - Cytokine expression and antigen-presenting capacity of 4F7+ dendritic cells derived from dermis, spleen, and lymph nodes
AU - Mohamadzadeh, Mansour
AU - Knop, Jürgen
AU - Aliani, Shahin
AU - Cruz, Ponciano D.
N1 - Funding Information:
Supported in part by the following NIH grants: AR-4-1940, RO1-AR35068, R29-AI31649 and PO1-DK42582. Recipient of a grant from the Deutsche Forschungsgemeinschaft
PY - 1997
Y1 - 1997
N2 - We took advantage of the recently generated 4F7 mAb, which recognizes an epitope expressed on dendritic cells (DC) from different tissues, to freshly isolate and positively sort for these cells and to characterize their cytokine pattern and antigen-presenting capacity in comparison with epidermal Langerhans cells (LC). RT-PCR and Northern blot analyses demonstrated constitutive mRNA expression of MIP-1γ, MIP-1α, C10, and IL-1β in both 4F7+ DC and LC. Lipopolysaccharide (LPS) treatment resulted in the upregulation of mRNA expression of all four cytokines and in a newly detected signal for TNFα. Immunoblot analysis showed constitutive secretion of MIP-1γ, with LPS treatment resulting in the upregulation of IL-1β production and in newly detected TNFα secretion. 4F7+ DC were also shown to express mRNA for the common γ chain receptor of IL-2 and for the receptor of IL-4. Finally, we demonstrated freshly isolated 4F7+ DC to be equivalent to freshly isolated LC in their capacity to present alloantigen in the mixed leukocyte reaction (MLR) and to process and present purified protein derivative (PPD) to Th1 and Th2 clones, We conclude that 4F7 is a useful marker for positively sorting DC from dermis, spleen, and lymph nodes. Regardless of tissue source, 4F7+ DC exhibit uniform cytokine and antigen-presenting capacity profiles that mimic the properties of freshly isolated epidermal LC.
AB - We took advantage of the recently generated 4F7 mAb, which recognizes an epitope expressed on dendritic cells (DC) from different tissues, to freshly isolate and positively sort for these cells and to characterize their cytokine pattern and antigen-presenting capacity in comparison with epidermal Langerhans cells (LC). RT-PCR and Northern blot analyses demonstrated constitutive mRNA expression of MIP-1γ, MIP-1α, C10, and IL-1β in both 4F7+ DC and LC. Lipopolysaccharide (LPS) treatment resulted in the upregulation of mRNA expression of all four cytokines and in a newly detected signal for TNFα. Immunoblot analysis showed constitutive secretion of MIP-1γ, with LPS treatment resulting in the upregulation of IL-1β production and in newly detected TNFα secretion. 4F7+ DC were also shown to express mRNA for the common γ chain receptor of IL-2 and for the receptor of IL-4. Finally, we demonstrated freshly isolated 4F7+ DC to be equivalent to freshly isolated LC in their capacity to present alloantigen in the mixed leukocyte reaction (MLR) and to process and present purified protein derivative (PPD) to Th1 and Th2 clones, We conclude that 4F7 is a useful marker for positively sorting DC from dermis, spleen, and lymph nodes. Regardless of tissue source, 4F7+ DC exhibit uniform cytokine and antigen-presenting capacity profiles that mimic the properties of freshly isolated epidermal LC.
KW - 4F7
KW - Antigen-presenting capacity
KW - Cytokines
KW - Dendritic cells
KW - Langerhans cells
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U2 - 10.1007/s004030050217
DO - 10.1007/s004030050217
M3 - Article
C2 - 9266019
AN - SCOPUS:0030865672
SN - 0340-3696
VL - 289
SP - 435
EP - 439
JO - Archives of Dermatological Research
JF - Archives of Dermatological Research
IS - 8
ER -