Cytogenetic evidence for gene amplification in mouse skin carcinogenesis

C. M. Aldaz, C. J. Conti, J. O'Connell, S. H. Yuspa, A. J. Klein-Szanto, Thomas J Slaga

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Using our recently developed methodology for cytogenetic evaluation of solid tumors, we analyzed the occurrence of double minutes (DM) in chemically induced mouse skin carcinomas and papillomas. These studies revealed that DM were observed in 10% of the squamous cell carcinomas and in none of the papillomas screened. In addition, we analyzed four cell lines derived from mouse skin papillomas and one spontaneously transformed mouse epidermal cell line. The presence of DM was a consistent feature in all the studied cell lines. In the papilloma cell lines, the degree that DM occurred correlated with the cell line's capacity to form malignant tumors in immunosuppressed mice. DM were also observed in direct chromosomal preparations from tumors induced by one of the papilloma cell lines. Homogeneously staining regions were also present in metaphases from the spontaneously transformed cell line. We have shown here, for the first time, DM and homogeneously staining regions in mouse skin tumors and transformed cells derived from mouse skin. Since DM and homogeneously staining regions are the cytogenetic equivalents of gene amplification, which is a mechanism of increased expression of normal or altered gene products, these findings may play a relevant role in mouse epidermal carcinogenesis.

Original languageEnglish (US)
Pages (from-to)3565-3568
Number of pages4
JournalCancer Research
Volume46
Issue number7
StatePublished - 1986
Externally publishedYes

Fingerprint

Gene Amplification
Cytogenetics
Carcinogenesis
Papilloma
Skin
Cell Line
Staining and Labeling
Neoplasms
Transformed Cell Line
Metaphase
Squamous Cell Carcinoma
Carcinoma
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Aldaz, C. M., Conti, C. J., O'Connell, J., Yuspa, S. H., Klein-Szanto, A. J., & Slaga, T. J. (1986). Cytogenetic evidence for gene amplification in mouse skin carcinogenesis. Cancer Research, 46(7), 3565-3568.

Cytogenetic evidence for gene amplification in mouse skin carcinogenesis. / Aldaz, C. M.; Conti, C. J.; O'Connell, J.; Yuspa, S. H.; Klein-Szanto, A. J.; Slaga, Thomas J.

In: Cancer Research, Vol. 46, No. 7, 1986, p. 3565-3568.

Research output: Contribution to journalArticle

Aldaz, CM, Conti, CJ, O'Connell, J, Yuspa, SH, Klein-Szanto, AJ & Slaga, TJ 1986, 'Cytogenetic evidence for gene amplification in mouse skin carcinogenesis', Cancer Research, vol. 46, no. 7, pp. 3565-3568.
Aldaz CM, Conti CJ, O'Connell J, Yuspa SH, Klein-Szanto AJ, Slaga TJ. Cytogenetic evidence for gene amplification in mouse skin carcinogenesis. Cancer Research. 1986;46(7):3565-3568.
Aldaz, C. M. ; Conti, C. J. ; O'Connell, J. ; Yuspa, S. H. ; Klein-Szanto, A. J. ; Slaga, Thomas J. / Cytogenetic evidence for gene amplification in mouse skin carcinogenesis. In: Cancer Research. 1986 ; Vol. 46, No. 7. pp. 3565-3568.
@article{f4651fb4a4d8439e948c51bf0616dd30,
title = "Cytogenetic evidence for gene amplification in mouse skin carcinogenesis",
abstract = "Using our recently developed methodology for cytogenetic evaluation of solid tumors, we analyzed the occurrence of double minutes (DM) in chemically induced mouse skin carcinomas and papillomas. These studies revealed that DM were observed in 10{\%} of the squamous cell carcinomas and in none of the papillomas screened. In addition, we analyzed four cell lines derived from mouse skin papillomas and one spontaneously transformed mouse epidermal cell line. The presence of DM was a consistent feature in all the studied cell lines. In the papilloma cell lines, the degree that DM occurred correlated with the cell line's capacity to form malignant tumors in immunosuppressed mice. DM were also observed in direct chromosomal preparations from tumors induced by one of the papilloma cell lines. Homogeneously staining regions were also present in metaphases from the spontaneously transformed cell line. We have shown here, for the first time, DM and homogeneously staining regions in mouse skin tumors and transformed cells derived from mouse skin. Since DM and homogeneously staining regions are the cytogenetic equivalents of gene amplification, which is a mechanism of increased expression of normal or altered gene products, these findings may play a relevant role in mouse epidermal carcinogenesis.",
author = "Aldaz, {C. M.} and Conti, {C. J.} and J. O'Connell and Yuspa, {S. H.} and Klein-Szanto, {A. J.} and Slaga, {Thomas J}",
year = "1986",
language = "English (US)",
volume = "46",
pages = "3565--3568",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "7",

}

TY - JOUR

T1 - Cytogenetic evidence for gene amplification in mouse skin carcinogenesis

AU - Aldaz, C. M.

AU - Conti, C. J.

AU - O'Connell, J.

AU - Yuspa, S. H.

AU - Klein-Szanto, A. J.

AU - Slaga, Thomas J

PY - 1986

Y1 - 1986

N2 - Using our recently developed methodology for cytogenetic evaluation of solid tumors, we analyzed the occurrence of double minutes (DM) in chemically induced mouse skin carcinomas and papillomas. These studies revealed that DM were observed in 10% of the squamous cell carcinomas and in none of the papillomas screened. In addition, we analyzed four cell lines derived from mouse skin papillomas and one spontaneously transformed mouse epidermal cell line. The presence of DM was a consistent feature in all the studied cell lines. In the papilloma cell lines, the degree that DM occurred correlated with the cell line's capacity to form malignant tumors in immunosuppressed mice. DM were also observed in direct chromosomal preparations from tumors induced by one of the papilloma cell lines. Homogeneously staining regions were also present in metaphases from the spontaneously transformed cell line. We have shown here, for the first time, DM and homogeneously staining regions in mouse skin tumors and transformed cells derived from mouse skin. Since DM and homogeneously staining regions are the cytogenetic equivalents of gene amplification, which is a mechanism of increased expression of normal or altered gene products, these findings may play a relevant role in mouse epidermal carcinogenesis.

AB - Using our recently developed methodology for cytogenetic evaluation of solid tumors, we analyzed the occurrence of double minutes (DM) in chemically induced mouse skin carcinomas and papillomas. These studies revealed that DM were observed in 10% of the squamous cell carcinomas and in none of the papillomas screened. In addition, we analyzed four cell lines derived from mouse skin papillomas and one spontaneously transformed mouse epidermal cell line. The presence of DM was a consistent feature in all the studied cell lines. In the papilloma cell lines, the degree that DM occurred correlated with the cell line's capacity to form malignant tumors in immunosuppressed mice. DM were also observed in direct chromosomal preparations from tumors induced by one of the papilloma cell lines. Homogeneously staining regions were also present in metaphases from the spontaneously transformed cell line. We have shown here, for the first time, DM and homogeneously staining regions in mouse skin tumors and transformed cells derived from mouse skin. Since DM and homogeneously staining regions are the cytogenetic equivalents of gene amplification, which is a mechanism of increased expression of normal or altered gene products, these findings may play a relevant role in mouse epidermal carcinogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0022651170&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022651170&partnerID=8YFLogxK

M3 - Article

C2 - 3708588

AN - SCOPUS:0022651170

VL - 46

SP - 3565

EP - 3568

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 7

ER -