Cytochemistry of Ca++-dependent adenosine triphosphatase (Ca-ATPase) in rat anterior pituitary cells

T. Soji, H. Nishizono, T. Yashiro, D. C. Herbert

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

In the present study, we demonstrate the localization of Ca++-ATPase in the anterior pituitary of the male rat. Ca++-ATPase was mainly distributed on the membrane system of the granular cells, which included the plasma membrane, the outer mitochondrial membrane, the enveloping membrane of secretory granules, the smooth endoplasmic reticulum and some components of the Golgi complex. No reaction product was detected on the membrane of the rough endoplasmic reticulum or that surrounding the lysosomes. A positive reaction was clearly observed on the membranes surrounding 'large' secretory granules, while that present on the membranes of the 'small' granules was comparatively weak. The cells which contained the 'large' granules were interpreted as growth hormone-secreting cells and those in which the 'small' granules were located as gonadotrophs. There were either no reaction or one that was barely detectable on the plasma membrane of the folliculo-stellate cells. These data along with our previous findings (Soji, 1982, 1984) suggest that the membranous enzymes are not uniformly distributed over all pituitary cells but rather are specific for a given cell population(s).

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalTissue and Cell
Volume23
Issue number1
DOIs
StatePublished - 1991

Keywords

  • anterior pituitary gland
  • ATP-ase
  • calcium ion
  • cytochemistry
  • gonadotroph
  • Pituitary
  • rat

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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