Cyclosporin A inhibits prostaglandin E2 production by fetal amnion cells in response to various stimuli

Samuel S. Edwin, D. Ware Branch, James R. Scott, Robert M. Silver, Donald J. Dudley, Murray D. Mitchell

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Use of cyclosporin A as part of an immunosuppressive regimen in pregnant transplant patients is not uncommon. Although successful pregnancies have been reported with the use of various immunosuppressive agents including cyclosporin A, the concern for fetal outcome still remains. Our purpose was to evaluate the effects of immunosuppressive cyclosporin A on prostaglandin E2 (PGE2) production by human fetal amnion. Amnion cells were isolated from term placentae obtained at elective cesarean section before the onset of labor. Cells were grown to confluence and then incubated for 16 hours with cyclosporin A (1-1000 ng/ml) in the presence and absence of interleukin 1β (IL-1β, 1 ng/ml), phorbol 12-myristate 13-acetate (PMA, 10-7 M) and ionomycin (0.5 μM). PGE2 was measured by radioimmunoassay and cellular protein determined. IL-1β, PMA and ionomycin all stimulated amnion cell PGE2 production as expected. However, these stimulatory actions were attenuated by at least 50% when cells were co-incubated with cyclosporin A (1000 ng/ml). Concentrations of cyclosporin A tested included the therapeutic range (250-1000 ng/ml). Our results indicate that cyclosporin A does not stimulate amnion cell PGE2 production and is probably unrelated to preterm labor and delivery in allograft recipients.

Original languageEnglish (US)
Pages (from-to)51-61
Number of pages11
JournalProstaglandins
Volume52
Issue number1
DOIs
StatePublished - Jul 1996
Externally publishedYes

Keywords

  • amnion cells
  • cyclosporin A
  • interleukin- 1β
  • ionomycin
  • phorbol 12-myristate 13-acetate
  • prostaglandin E

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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