Cyclic pressure affects osteoblast functions pertinent to osteogenesis

Jiro Nagatomi, Bernard P. Arulanandam, Dennis W. Metzger, Alain Meunier, Rena Bizios

Research output: Contribution to journalArticlepeer-review

47 Scopus citations


In an attempt to elucidate the cellular/molecular correlations between mechanical stimuli and new bone formation, the present in vitro study used a custom-made laboratory setup and examined the effects of cyclic pressure on select functions of osteoblasts pertinent to osteogenesis. The results demonstrated that, compared to controls (no pressure), mRNA expression for type-I collagen (the main constituent of the organic phase of bone) was enhanced when osteoblasts were exposed to cyclic pressure (10-40 kPa at 1.0 Hz) for 1 h daily for up to 19 consecutive days. In addition, compared to controls, both deposition of collagen and accumulation of calcium (one of the major components of the inorganic phase of bone) increased significantly (p <0.05) following exposure of osteoblast cultures to cyclic pressure for 19 days. Since the amounts of total DNA in controls and in osteoblast cultures exposed to cyclic pressure were similar at all time points tested, it was concluded that increased collagen and calcium concentrations in cultures resulted from enhanced osteoblast function (and not from increased number of cells); the presence of increased amounts of collagen affected the subsequent increased accumulation of calcium. These results provide evidence that daily exposure of cyclic pressure for various time periods (up to 19 days) affect osteoblast functions pertinent to bone formation.

Original languageEnglish (US)
Pages (from-to)917-923
Number of pages7
JournalAnnals of Biomedical Engineering
Issue number8
StatePublished - 2003


  • Bone formation
  • Calcium
  • mRNA
  • Type-I collagen

ASJC Scopus subject areas

  • Biomedical Engineering


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