Cyclic appearance of defective interfering particles of herpes simplex virus and the concomitant accumulation of early polypeptide VP175

Byron K. Murray; Nilambar Biswal; Jack B. Bookout; Robert E. Lanford; Richard J. Courtney; Joseph L. Melnick

doi:10.1159/000149894

Cyclic appearance of defective interfering particles of herpes simplex virus and the concomitant accumulation of early polypeptide VP175

Byron K. Murray, Nilambar Biswal, Jack B. Bookout, Robert E. Lanford, Richard J. Courtney, Joseph L. Melnick

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Serial passage of undiluted herpes simplex virus types 1 and 2 resulted in cyclic production of infectious and defective virions. Defective virus production was characterized by the appearance of a new species of viral DNA with a higher buoyant density in CsCl than standard viral DNA. Measurement of the infectivity titer and DNA synthesis revealed that the defective particles interfered with the replication of standard virions and stimulated the overproduction of a large molecular weight (175,000 daltons) polypeptide.

Original language	English (US)
Pages (from-to)	173-184
Number of pages	12
Journal	Intervirology
Volume	5
Issue number	3-4
DOIs	https://doi.org/10.1159/000149894
State	Published - 1975

Keywords

Defective herpes simplex virions
High-density DNA
Interference
Polypeptide VP175

ASJC Scopus subject areas

Virology
Infectious Diseases

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10.1159/000149894

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Cyclic appearance of defective interfering particles of herpes simplex virus and the concomitant accumulation of early polypeptide VP175. / Murray, Byron K.; Biswal, Nilambar; Bookout, Jack B.; Lanford, Robert E.; Courtney, Richard J.; Melnick, Joseph L.

In: Intervirology, Vol. 5, No. 3-4, 1975, p. 173-184.

Research output: Contribution to journal › Article › peer-review

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AU - Courtney, Richard J.

AU - Melnick, Joseph L.

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AB - Serial passage of undiluted herpes simplex virus types 1 and 2 resulted in cyclic production of infectious and defective virions. Defective virus production was characterized by the appearance of a new species of viral DNA with a higher buoyant density in CsCl than standard viral DNA. Measurement of the infectivity titer and DNA synthesis revealed that the defective particles interfered with the replication of standard virions and stimulated the overproduction of a large molecular weight (175,000 daltons) polypeptide.

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KW - Polypeptide VP175

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Cyclic appearance of defective interfering particles of herpes simplex virus and the concomitant accumulation of early polypeptide VP175

Abstract

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