Cyclic appearance of defective interfering particles of herpes simplex virus and the concomitant accumulation of early polypeptide VP175

Byron K. Murray, Nilambar Biswal, Jack B. Bookout, Robert E. Lanford, Richard J. Courtney, Joseph L. Melnick

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    25 Scopus citations

    Abstract

    Serial passage of undiluted herpes simplex virus types 1 and 2 resulted in cyclic production of infectious and defective virions. Defective virus production was characterized by the appearance of a new species of viral DNA with a higher buoyant density in CsCl than standard viral DNA. Measurement of the infectivity titer and DNA synthesis revealed that the defective particles interfered with the replication of standard virions and stimulated the overproduction of a large molecular weight (175,000 daltons) polypeptide.

    Original languageEnglish (US)
    Pages (from-to)173-184
    Number of pages12
    JournalIntervirology
    Volume5
    Issue number3-4
    DOIs
    Publication statusPublished - Jan 1 1975

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    Keywords

    • Defective herpes simplex virions
    • High-density DNA
    • Interference
    • Polypeptide VP175

    ASJC Scopus subject areas

    • Virology
    • Infectious Diseases

    Cite this

    Murray, B. K., Biswal, N., Bookout, J. B., Lanford, R. E., Courtney, R. J., & Melnick, J. L. (1975). Cyclic appearance of defective interfering particles of herpes simplex virus and the concomitant accumulation of early polypeptide VP175. Intervirology, 5(3-4), 173-184. https://doi.org/10.1159/000149894