The regulation of cyclic adenosine 3',5'-monophosphate (cyclic AMP) formation in rat spinal cord tissue slices was studied. Significant stimulation of accumulation of cyclic AMP was produced by various amines (norepinephrine, epinephrine, isoproterenol, dopamine, serotonin and phenylephrine) and adenosine. Histamine, cholinomimetics, and various excitatory and inhibitory amino acids were without effect, as well as prostaglandin E1 and F1α The norepinephrine response was blocked partially by either alpha or beta receptor antagonists and blocked completely by combined incubation with the two antagonists. Alpha and beta agonist-induced increases in cyclic AMP were blocked completely by the respective adrenergic antagonists. Potentiation of the norepinephrine response by the phosphodiesterase inhibitor RO 20-1724 suggests that cyclic AMP formation in the spinal cord is linked to adrenergic receptor activation and this system may play a role in the regulation of neuronal function in spinal cord.
- alpha- and beta-adrenergic receptors
- cyclic AMP
- rat spinal cord
- tissue slices
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience