Cyclic AMP formation in rat spinal cord tissue slices

D. J. Jones; L. F. Mckenna

doi:10.1016/0028-3908(80)90042-8

Cyclic AMP formation in rat spinal cord tissue slices

D. J. Jones, L. F. Mckenna

Anesthesiology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The regulation of cyclic adenosine 3',5'-monophosphate (cyclic AMP) formation in rat spinal cord tissue slices was studied. Significant stimulation of accumulation of cyclic AMP was produced by various amines (norepinephrine, epinephrine, isoproterenol, dopamine, serotonin and phenylephrine) and adenosine. Histamine, cholinomimetics, and various excitatory and inhibitory amino acids were without effect, as well as prostaglandin E₁ and F_1α The norepinephrine response was blocked partially by either alpha or beta receptor antagonists and blocked completely by combined incubation with the two antagonists. Alpha and beta agonist-induced increases in cyclic AMP were blocked completely by the respective adrenergic antagonists. Potentiation of the norepinephrine response by the phosphodiesterase inhibitor RO 20-1724 suggests that cyclic AMP formation in the spinal cord is linked to adrenergic receptor activation and this system may play a role in the regulation of neuronal function in spinal cord.

Original language	English (US)
Pages (from-to)	669-674
Number of pages	6
Journal	Neuropharmacology
Volume	19
Issue number	7
DOIs	https://doi.org/10.1016/0028-3908(80)90042-8
State	Published - Jul 1980

Keywords

alpha- and beta-adrenergic receptors
cyclic AMP
rat spinal cord
tissue slices

ASJC Scopus subject areas

Pharmacology
Cellular and Molecular Neuroscience

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title = "Cyclic AMP formation in rat spinal cord tissue slices",

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AB - The regulation of cyclic adenosine 3',5'-monophosphate (cyclic AMP) formation in rat spinal cord tissue slices was studied. Significant stimulation of accumulation of cyclic AMP was produced by various amines (norepinephrine, epinephrine, isoproterenol, dopamine, serotonin and phenylephrine) and adenosine. Histamine, cholinomimetics, and various excitatory and inhibitory amino acids were without effect, as well as prostaglandin E1 and F1α The norepinephrine response was blocked partially by either alpha or beta receptor antagonists and blocked completely by combined incubation with the two antagonists. Alpha and beta agonist-induced increases in cyclic AMP were blocked completely by the respective adrenergic antagonists. Potentiation of the norepinephrine response by the phosphodiesterase inhibitor RO 20-1724 suggests that cyclic AMP formation in the spinal cord is linked to adrenergic receptor activation and this system may play a role in the regulation of neuronal function in spinal cord.

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KW - tissue slices

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Cyclic AMP formation in rat spinal cord tissue slices

Abstract

Keywords

ASJC Scopus subject areas

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