Cyclic AMP-dependent modification of gonad-selective TAFII105 in a human ovarian granulosa cell line

Yimin Wu, Yunzhe Lu, Yanfen Hu, Rong Li

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In response to gonadotropins, the elevated level of intracellular-cyclic AMP (cAMP) in ovarian granulosa cells triggers an ordered activation of multiple ovarian genes, which in turn promotes various ovarian functions including folliculogenesis and steroidogenesis. Identification and characterization of transcription factors that control ovarian gene expression are pivotal to the understanding of the molecular basis of the tissue-specific gene regulation programs. The recent discovery of the mouse TATA binding protein (TBP)-associated factor 105 (TAFII105) as a gonad-selective transcriptional co-activator strongly suggests that general transcription factors such as TFIID may play a key role in regulating tissue-specific gene expression. Here we show that the human TAFII105 protein is preferentially expressed in ovarian granulosa cells. We also identified a novel TAFII105 mRNA isoform that results from alternative exon inclusion and is predicted to encode a dominant negative mutant of TAFII105. Following stimulation by the adenylyl cyclase activator forskolin, TAF II105 in granulosa cells undergoes rapid and transient phosphorylation that is dependent upon protein kinase A (PKA). Thus, our work suggests that pre-mRNA processing and post-translational modification represent two important regulatory steps for the gonad-specific functions of human TAFII105.

Original languageEnglish (US)
Pages (from-to)751-759
Number of pages9
JournalJournal of Cellular Biochemistry
Volume96
Issue number4
DOIs
StatePublished - Nov 1 2005
Externally publishedYes

Fingerprint

Granulosa Cells
Gonads
Gene expression
Cyclic AMP
Cells
Cell Line
TATA-Binding Protein Associated Factors
Transcription Factor TFIID
General Transcription Factors
Tissue
RNA Isoforms
Gene Expression
Phosphorylation
RNA Precursors
Colforsin
Post Translational Protein Processing
Cyclic AMP-Dependent Protein Kinases
Gonadotropins
Adenylyl Cyclases
Genes

Keywords

  • Alternative splicing
  • cAMP
  • Ovarian granulosa cells
  • Phopshorylation
  • TAF105

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Cyclic AMP-dependent modification of gonad-selective TAFII105 in a human ovarian granulosa cell line. / Wu, Yimin; Lu, Yunzhe; Hu, Yanfen; Li, Rong.

In: Journal of Cellular Biochemistry, Vol. 96, No. 4, 01.11.2005, p. 751-759.

Research output: Contribution to journalArticle

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