Curcumin inhibits the classical and the alternate pathways of complement activation

Amod P. Kulkarni; Yohannes T. Ghebremariam; Girish J. Kotwal

doi:10.1196/annals.1352.007

Curcumin inhibits the classical and the alternate pathways of complement activation

Amod P. Kulkarni, Yohannes T. Ghebremariam, Girish J. Kotwal

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Curcumin (Cur), the golden yellow phenolic compound in turmeric, is well studied for its medicinal properties. In the current investigation, Cur dissolved using sodium hydroxide solution (CurNa) was tested for in vitro complement inhibitory activity and compared with rosmarinic acid (RA) and quercetin (Qur) dissolved using sodium hydroxide (RANa and QurNa, respectively) and the vaccinia virus complement control protein (VCP). The comparative study indicated that CurNa inhibited the classical complement pathway dose dependently (IC₅₀ = 404 μM). CurNa was more active than RANa, but less active than QurNa. VCP was about 2,212, 2,786, and 4,520 times more active than QurNa, CurNa, and RANa, respectively. Further study revealed that CurNa dose dependently inhibited zymosan-induced activation of the alternate pathway of complement activation.

Original language	English (US)
Pages (from-to)	100-112
Number of pages	13
Journal	Annals of the New York Academy of Sciences
Volume	1056
DOIs	https://doi.org/10.1196/annals.1352.007
State	Published - 2005
Externally published	Yes

Keywords

Alternate pathway (AP)
Bb
C3b
Classical pathway (CP)
Complement
Curcumin
Hemolysis
Quercetin
Rosmarinic acid
VCP

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Neuroscience
History and Philosophy of Science

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10.1196/annals.1352.007

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title = "Curcumin inhibits the classical and the alternate pathways of complement activation",

abstract = "Curcumin (Cur), the golden yellow phenolic compound in turmeric, is well studied for its medicinal properties. In the current investigation, Cur dissolved using sodium hydroxide solution (CurNa) was tested for in vitro complement inhibitory activity and compared with rosmarinic acid (RA) and quercetin (Qur) dissolved using sodium hydroxide (RANa and QurNa, respectively) and the vaccinia virus complement control protein (VCP). The comparative study indicated that CurNa inhibited the classical complement pathway dose dependently (IC50 = 404 μM). CurNa was more active than RANa, but less active than QurNa. VCP was about 2,212, 2,786, and 4,520 times more active than QurNa, CurNa, and RANa, respectively. Further study revealed that CurNa dose dependently inhibited zymosan-induced activation of the alternate pathway of complement activation.",

keywords = "Alternate pathway (AP), Bb, C3b, Classical pathway (CP), Complement, Curcumin, Hemolysis, Quercetin, Rosmarinic acid, VCP",

author = "Kulkarni, {Amod P.} and Ghebremariam, {Yohannes T.} and Kotwal, {Girish J.}",

year = "2005",

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T1 - Curcumin inhibits the classical and the alternate pathways of complement activation

AU - Kulkarni, Amod P.

AU - Ghebremariam, Yohannes T.

AU - Kotwal, Girish J.

PY - 2005

Y1 - 2005

N2 - Curcumin (Cur), the golden yellow phenolic compound in turmeric, is well studied for its medicinal properties. In the current investigation, Cur dissolved using sodium hydroxide solution (CurNa) was tested for in vitro complement inhibitory activity and compared with rosmarinic acid (RA) and quercetin (Qur) dissolved using sodium hydroxide (RANa and QurNa, respectively) and the vaccinia virus complement control protein (VCP). The comparative study indicated that CurNa inhibited the classical complement pathway dose dependently (IC50 = 404 μM). CurNa was more active than RANa, but less active than QurNa. VCP was about 2,212, 2,786, and 4,520 times more active than QurNa, CurNa, and RANa, respectively. Further study revealed that CurNa dose dependently inhibited zymosan-induced activation of the alternate pathway of complement activation.

AB - Curcumin (Cur), the golden yellow phenolic compound in turmeric, is well studied for its medicinal properties. In the current investigation, Cur dissolved using sodium hydroxide solution (CurNa) was tested for in vitro complement inhibitory activity and compared with rosmarinic acid (RA) and quercetin (Qur) dissolved using sodium hydroxide (RANa and QurNa, respectively) and the vaccinia virus complement control protein (VCP). The comparative study indicated that CurNa inhibited the classical complement pathway dose dependently (IC50 = 404 μM). CurNa was more active than RANa, but less active than QurNa. VCP was about 2,212, 2,786, and 4,520 times more active than QurNa, CurNa, and RANa, respectively. Further study revealed that CurNa dose dependently inhibited zymosan-induced activation of the alternate pathway of complement activation.

KW - Alternate pathway (AP)

KW - Bb

KW - C3b

KW - Classical pathway (CP)

KW - Complement

KW - Curcumin

KW - Hemolysis

KW - Quercetin

KW - Rosmarinic acid

KW - VCP

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JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -

Curcumin inhibits the classical and the alternate pathways of complement activation

Abstract

Keywords

ASJC Scopus subject areas

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