Curcumin ameliorates alveolar bone destruction of experimental periodontitis by modulating osteoclast differentiation, Activation and function

Lian Ping Mau, Wan Chien Cheng, Jen Kun Chen, Yi Shing Shieh, David L Cochran, Ren Yeong Huang

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

This study evaluated as to whether curcumin (CCM) could ameliorate alveolar bone destruction in vivo and dissect its mechanism in vitro. The tartrate-resistant acid phosphatase (TRAP) activity, TRAP staining, quantitative RT-PCR, gelatin zymography, actin-ring formation, and pits formation assay were used to analyse the effect of CCM on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclast differentiation, activation, and function. Porphyromonas gingivalis LPS- and ligature-induced experimental periodontitis were established to evaluate the therapeutic benefits of CCM in vivo. The results demonstrated that CCM dose dependently diminishes RANKL-induced osteoclast differentiation, and osteoclastic specific genes expression accompanied by a significant attenuation of actin-ring and resorptive pits formation in vitro. Moreover, CCM reduces alveolar bone destruction, decreases TRAP-positive and polymorphonuclear cells infiltration, and suppresses myeloperoxidase activity in vivo. Thus, CCM reduces inflammatory bone loss in vivo by modulating RANKL-mediated osteoclast differentiation, activation, and function.

Original languageEnglish (US)
Pages (from-to)243-256
Number of pages14
JournalJournal of Functional Foods
Volume22
DOIs
StatePublished - Apr 1 2016

Keywords

  • Bone resorption
  • Curcumin
  • Osteoclastogenesis
  • Periodontal disease

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Fingerprint Dive into the research topics of 'Curcumin ameliorates alveolar bone destruction of experimental periodontitis by modulating osteoclast differentiation, Activation and function'. Together they form a unique fingerprint.

  • Cite this