Culture of Human Gastrointestinal Epithelial Cells

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83 Scopus citations

Abstract

Human gastrointestinal (GI) epithelial cells and tissues have been propagated in vitro for several months. Viability of typical epithelial cells with minimal contamination by fibroblasts was best achieved by mechanical harvesting instead of using enzymes for dissociation or subculture. Individual cells generally adhered poorly to glass and plastic although they did attach to some coated substrates. Although standard formulations of culture base media were suitable, there was a continuous requirement for conditioned medium and cultures were readily propagated in suspension. Cultures had a propensity to grow as “islands” of epithelial cells consisting of central dividing regions and external “differentiated” regions. As yet undefined factors from yeast and brain (or pituitary) extracts were required for optimal growth.

Original languageEnglish (US)
Pages (from-to)12-15
Number of pages4
JournalProceedings of the Society for Experimental Biology and Medicine
Volume174
Issue number1
DOIs
StatePublished - Oct 1983
Externally publishedYes

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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