TY - JOUR
T1 - Culture and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) Proven Mycobacterium Tuberculosis Endophthalmitis
T2 - A Case Series
AU - Rishi, Ekta
AU - Rishi, Pukhraj
AU - Therese, K. Lily
AU - Ramasubban, Gayathri
AU - Biswas, Jyotirmay
AU - Sharma, Tarun
AU - Bhende, Pramod
AU - Susvar, Pradeep
AU - Agarwal, Mamta
AU - George, Amala Elizabeth
AU - Delhiwala, Kushal
AU - Sharma, Vishal Rajan
N1 - Publisher Copyright:
© Taylor & Francis Group, LLC.
PY - 2018/2/17
Y1 - 2018/2/17
N2 - Purpose: To report early confirmation of Mycobacterium tuberculosis (MTB) endophthalmitis by detection of 85B mRNA in vitreous by a reverse transcriptase polymerase chain reaction (RT-PCR) technique. Methods: Retrospective, interventional case series of 5 patients with MTB endogenous endophthalmitis. Vitreous aspirate was subjected to Ziehl-Neelsen (ZN) staining, BACTEC MicroMGIT culture, RT-PCR targeting the 85B gene, real-time PCR targeting the IS6110 region, and nested PCR targeting the MPB64 gene and IS6110 region. Correlation between detection of MTB RNA, culture positivity, and ZN staining was studied. Results: Five patients with endophthalmitis with no history of tuberculosis revealed acid-fast bacilli on ZN staining of vitreous. RT-PCR detected 85B RNA within 24 h. Culture for MTB was positive in 3/5 patients after 1 month. None of the eyes recovered any useful vision. Conclusions: RT-PCR can detect viable MTB RNA and provide evidence of active infection much earlier than culture.
AB - Purpose: To report early confirmation of Mycobacterium tuberculosis (MTB) endophthalmitis by detection of 85B mRNA in vitreous by a reverse transcriptase polymerase chain reaction (RT-PCR) technique. Methods: Retrospective, interventional case series of 5 patients with MTB endogenous endophthalmitis. Vitreous aspirate was subjected to Ziehl-Neelsen (ZN) staining, BACTEC MicroMGIT culture, RT-PCR targeting the 85B gene, real-time PCR targeting the IS6110 region, and nested PCR targeting the MPB64 gene and IS6110 region. Correlation between detection of MTB RNA, culture positivity, and ZN staining was studied. Results: Five patients with endophthalmitis with no history of tuberculosis revealed acid-fast bacilli on ZN staining of vitreous. RT-PCR detected 85B RNA within 24 h. Culture for MTB was positive in 3/5 patients after 1 month. None of the eyes recovered any useful vision. Conclusions: RT-PCR can detect viable MTB RNA and provide evidence of active infection much earlier than culture.
KW - Acid fast bacilli
KW - Mycobacterium tuberculosis
KW - RT-PCR
KW - endophthalmitis
KW - vitrectomy
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U2 - 10.1080/09273948.2016.1207786
DO - 10.1080/09273948.2016.1207786
M3 - Article
C2 - 27598944
AN - SCOPUS:84986222477
SN - 0927-3948
VL - 26
SP - 220
EP - 227
JO - Ocular Immunology and Inflammation
JF - Ocular Immunology and Inflammation
IS - 2
ER -