Cultivation of mammalian cells in heat-sealable pouches that are permeable to carbon dioxide

Robert J. Klebe, Shauna Lyn, Victoria L. Magnuson, Gustavo Zardeneta

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Petri plates, 96-well plates, and other unsealed culture vessels are the chief cause of air-borne contamination of cell cultures. In this study, heat-sealable plastic pouches that are permeable to CO2 and other gases are used as a means to avoid contamination. Several applications of heat-sealable pouches are described. First, petri plates can simply be sealed in a wide variety of plastic films that are permeable to CO2. Second, a few CO2-permeable plastic films can be used directly as substrata for mammalian cell growth and can also be cut with a simple hole punch for the isolation of clones. Third, one can grow cells in chemically sterilized carbonic acid solutions and thereby avoid the use of a CO2 incubator entirely.

Original languageEnglish (US)
Pages (from-to)316-319
Number of pages4
JournalExperimental Cell Research
Volume188
Issue number2
DOIs
StatePublished - Jun 1990

ASJC Scopus subject areas

  • Cell Biology

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